通过添加10μL的43 Laemmli样品缓冲液终止孵育。煮沸5分钟后,将混合物在16%凝胶上进行SDS-聚丙烯酰胺凝胶电泳。用考马斯亮蓝染色凝胶,然后干燥。切除对应于组蛋白2型的条带,并测量放射性[1]。 细胞实验HeLa细胞以每3.5cm培养皿3×104个细胞的密度接种。在10mM胸苷存在下,将细胞在含有10%FBS的DMEM中培养16小时。
分别在0,5,10和20分钟采集7μl等分样,与等体积2×Laemmli样本缓冲液混合,然后进行SDS-PAGE。使用考马斯亮蓝对凝胶进行染色,烘干,然后通过Bioimage Analyzer BAS2000分析。获得抑制50% p160ROCK活性所需的Y-27632浓度(IC50值)。 细胞实验 细胞系N/A 浓度N/A...
分别在0,5,10和20分钟采集7μl等分样,与等体积2×Laemmli样本缓冲液混合,然后进行SDS-PAGE。使用考马斯亮蓝对凝胶进行染色,烘干,然后通过Bioimage Analyzer BAS2000分析。获得抑制50% p160ROCK活性所需的Y-27632浓度(IC50值)。 细胞实验 细胞系N/A 浓度N/A...
大包装询价添加到收藏夹比较SDS中文版DATASHEET 查看相关系列 Cell Cycle/DNA Damage (2603)Cytoskeleton (499)ROCK (71)Stem Cell/Wnt (1015)TGF-beta/Smad (303) Skip to the end of the images gallery Skip to the beginning of the images gallery ...
资料来源 SDS 样本 Sigma-Aldrich www.sigmaaldrich.com/catalog/Lookup.do?N5=All&N3=mode+matchpartialmax&N1=S_ID&ST=RS&N25=0&F=PR&D7=0&N4=129830-38-2 浏览或下载 Matrix www.matrixscientific.com/media/msds/14/37/99/MxMSDS_143799.pdf 浏览或下载 Cayman www.caymanchem.com/msdss/1000558...
volume of 30 μL. Incubation is terminated by the addition of 10 μL of 43 Laemmli sample buffer. After boiling for 5 min, the mixture is subjected to SDS-polyacrylamide gel electrophoresis on a 16% gel. The gel is stained with Coomassie Brilliant Blue, and then dried. The bands ...
Y-27632 DIHYDROCHLORIDE (CAS 146986-50-7) information, including chemical properties, structure, melting point, boiling point, density, formula, molecular weight, uses, prices, suppliers, SDS and more, available at Chemicalbook.
30 μL. Incubation is terminated by the addition of 10 μL of 43 Laemmli sample buffer. After boiling for 5 min, the mixture is subjected to SDS-polyacrylamide gel electrophoresis on a 16% gel. The gel is stained with Coomassie Brilliant Blue and then dried. The bands corresponding to ...
大包装询价 添加到收藏夹 比较 SDS中文版 DATASHEET 查看相关系列 Apoptosis (1463) Cell Cycle/DNA Damage (2603) Cytoskeleton (499) protein kinase C epsilon Inhibitor (5) protein kinase N2 Inhibitor (2) Rho associated coiled-coil containing protein kinase 1 Inhibitor (17) Rho associated coiled-coi...
After boiling for 5 min, the mixture is subjected to SDS-polyacrylamide gel electrophoresis on a 16% gel. The gel is stained with Coomassie Brilliant Blue, and then dried. The bands corresponding to histone type 2 are excised, and the radioactivity is measured[1]. 体外活性 方法:人诱导多能...