Secondly, the isomerase pathway, which is mainly found in bacteria, is a one-step reaction catalysed by XI. This enzyme directly converts xylose to xylulose without requiring cofactors [16, 17]. However, XI activity is inhibited by xylitol formation by the unspecific endogenous NADPH-dependent ...
xylose isomerase XK: xylulokinase X5P: xylulose-5-phosphate XR: xylose reductase ZWF: glucose-6-phosphate dehydrogenase.References Hahn-Hägerdal B, Galbe M, Gorwa-Grauslund MF, Lidén G, Zacchi G: Bio-ethanol - the fuel of tomorrow from the residues of today. Trends Biotechnol. ...
The engineered strain Xsp8 was introduced with two heterologous genes from Streptomyces: xylA, encoding xylose isomerase, and xylB, encoding xylulokinase. We further demonstrated that in addition to the introduction and the concomitant expression of heterologous xylA and xylB genes, there is another ...
isomerase, and Weimberg–Dahms pathways [56]. For example,E. coliis a robust and well-studied xylose scavenger [56,62], which could metabolize xylose by the isomerase pathway; however,S. stipites[56,61] could utilize oxidoreductase pathway for the metabolism of xylose. In the xylose isomera...
Xylose isomerase (XI) catalyses the isomerisation of xylose to xylulose in bacteria and some fungi. Currently, only a limited number of XI genes have been functionally expressed in Saccharomyces cerevisiae, the microorganism of choice for lignocellulosic
Functional expression of a bacterial xylose isomerase in Saccharomyces cerevisiae. Appl Environ Microbiol. 2009;75:2304–11. Article CAS Google Scholar Karhumaa K, Wiedemann B, Hahn-Hägerdal B, Boles E, Gorwa-Grauslund MF. Co-utilization of l-arabinose and d-xylose by laboratory and ...
The yeastS. cerevisiae, a generally recognised as safe microorganism, is naturally incapable of efficient xylose metabolism. Currently, there are two major successfully implemented strategies for xylose utilisation byS. cerevisiaevia the oxidoreductase (XR/XDH) pathway and the xylose isomerase (XI) pathw...
Xylose isomerase (EC 5.3.1.5) is required for the first reaction of xylose utilization, converting D-xylose into D-xylulose. This enzyme, a product of thexylAgene, was characterized in detail inL. lactis[29], and its orthologs are present in many bacteria includingB. subtilis. However, analy...
An16g05420 (pgi1) Glucose-6-phosphate isomerase 1.63 1.39 0.74 1.18 ND ND ND ND Galactose pathway An02g03590 (gal7) UDP-galactose-pyrophosphorylase 1.85 1.98 2.20 2.25 ND ND ND ND An14g03820 (gal10) UDP-glucose-4-epimerase 1.38 1.60 ND ND ND ND ND ND An16g04160 (gal1) Galactokinase...
Xylose isomerase (EC 5.3.1.5) is required for the first reaction of xylose utilization, converting D-xylose into D-xylulose. This enzyme, a product of thexylAgene, was characterized in detail inL. lactis[29], and its orthologs are present in many bacteria includingB. subtilis. However, analy...