RNA sequencing (RNA-seq) libraries were prepared using the Illumina TruSeq Stranded mRNA Library Prep Kit. Paired-end RNA-seq were performed with an Illumina NextSeq sequencing instrument (Helixio, France). i-BLESS Samples for i-BLESS analysis were prepared as described46with minor modifications....
英文名称:Topoisomerase I Assay Kit (62.5ug SC DNA) 欣博盛生物科技有限公司 联系电话:4006-800-892 电子邮件:info@neobioscience.com CB指数:58 网址:www.nbs-bio.com 相关信息:产品目录(6207)用户评价 英文名称:Topoisomerase I Assay Kit (62.5ug SC DNA) ...
DNA Topoisomerase I 催化4种反应: 1. 使环状超螺旋分子的超螺旋解开。 2. 在单链环状DNA分子内形成绳结 (Knotting) 和解开绳结 (Unknotting)。 3. 催化具有互补碱基序列的环状单链DNA形成双链闭环状DNA分子。 4. 2个环状双链DNA分子之中的一个存在DNA链的切口时,发生两个分子的连结反应 (Catenation) 或者...
FACS-based H2AX phosphorylation assay kit (Millipore, catalogue # 17–344) was used to stain gH2AX in CH12F3-2A cells, essentially as described by the manufacturer. The cells were analysed on a Becton-Dickinson FACS-Calibur flow cytometer. Construction of plasmids. The human SMARCA4 cDNA was ...
Luciferase activity of HeLa LTR-Luc cells was measured using the Luciferase Assay System kit (Promega) according to the manufacturer instructions and quantified on a FLUOstar OPTIMA (BMG LABTECH) (Fig.1) or a CLARIOstar (BMG LABTECH) (Fig.6). ...
RNA-seq. RNA sequencing (RNA-seq) libraries were prepared using the Illumina TruSeq Stranded mRNA Library Prep Kit. Paired-end RNA-seq were performed with an Illumina NextSeq sequencing instrument (Helixio, France). i-BLESS. Samples for i-BLESS analysis were prepared as described46 with minor...
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To elucidate the impact of circ 0008896 on ox-LDL-induced damage to human aortic endothelial cells (HAECs), a multifaceted functional approach was employed, including analysis by enzyme-linked immunosorbent assay (ELISA), cell counting kit-8 (CCK-8) measurements, 5-ethynyl-2'-deoxyuridine (EdU)...
It was observed that 6a also induced apoptosis by caspases 3/7 activity using a Capsase-Glo-3/7 assay kit and inhibited TOP1. A possible binding mode of 6a in a complex with TOP1-DNA was proposed by docking and molecular dynamics studies. In addition, 6a was pred...
TOP1 ICE (in vivo complex of enzyme) assay Human Topoisomerase 1 ICE Assay Kit (TopoGEN, Cat. No. TG1020-1) was used to isolate protein–DNA samples which contain TOP1–DNA covalent complex (TOP1cc) for slot blot analysis. The isolation was performed following the manufacturer’s guideline...