Here, we describe mass spectrometric analysis of CTCs from a breast cancer xenograft mouse model, including procedures comprising CTC enrichment, separation of different CTC subpopulations, and their quantitative proteomic assessment. This protocol aims to facilitate the identification of protein content ...
Discussion In the present study, we analysed the impact of pemetrexed on the gut microbiota composition, the colon mucosal integrity and inflammation in a mouse xenograft model of a human lung adenocarcinoma. The tumor graft induced variations in faecal microbiota composition, whereas pemetrexed ...
Herein, anin vivoselection procedure is described that enabled the identification of prostate tumour recognising DNA aptamers. An orthotopic xenograft mouse model was applied during the selection and validation procedure to secure tissue penetration and recognition of the relevant microenvironment of the tum...
properties (1,4). Additional immunodeficient mouse strains are also available for human tumor experimentation. The combination of the nude and beige mutations produced athymic mice with reduced NK cell activity. The triple deficient beige (bg)/nude(nu)/xidmouse is functionally depleted of T and B...
The mouse DP is androgen sensitive and considered homologous to the human peripheral zone, while the mouse AP has less clinical significance as it is similar to the human central zone [9]. To the authors’ knowledge, there is no comprehensive protocol describing microscope-guided orthotopic ...
of cells within the mouse. However, the most crucial disappointment with mouse xenograft models has been the consistent failure, in humanclinical trials, of drugs selected based on preclinical studies on these models [85]. Therefore, alternatives to the mouse xenograft model have been sought. One...
www.nature.com/scientificreports OPEN 3′-hydroxy-3,4,5,4′- tetramethoxystilbene, the metabolite of resveratrol analogue received: 29 February 2016 DMU-212, inhibits ovarian canceraccepted:11August2016 Published:02September2016 cell growth in vitro and in a mice xenograft model Hanna Piotrowska-...
Liver metastasis is a recalcitrant disease that usually leads to death of the patient. The present study established a unique patient-derived orthotopic xenograft (PDOX) nude mouse model of a highly aggressive liver metastasis of colon cancer. The aim of the present study was to demonstrate proof...
The results demonstrate that WSZG exerts potential therapeutic effects in a mouse xenograft model of breast cancer bone metastases, which are partially mediated by weakening the interaction between BMSCs and breast cancer cells in the tumor microenvironment. Keywords: breast cancer; bone metastases; ...
Establishment of the Subcutaneous Pancreatic Cancer Cell Mouse Xenograft Model and Treatment Protocol. Antitumor Activity of Intratumoral Ethanol Injection in an Orthotopic Pancreatic Cancer Cell Mouse Xenograft Model Among these, collagen sponge is the faster to be resorbed, while xenograft demonstrated to...