X-ray crystallography was used to determine the structure of a fragment of CD46 and electron microscopy was used to determine the low resolution structure of a complex of transferrin (Tf) and the transferrin receptor (TfR).; CD46 is complement regulatory protein with an extracellular region ...
The incident beam is elastically scattered by the electrons of the target atoms into many specific directions which are characteristic of the atomic and molecular structure of the crystals, thus allowing a direct and quantitative access to the crystallography of the sample. In some beam-lines, the...
Because of this lack of contrast, X-ray-based techniques may not be good for soft-tissue and organ imaging if no contrast agent is used. View chapterExplore book 3D Structural Determination of Macromolecules Using X-ray Crystallography Methods Mutharasappan Nachiappan, ... Jeyaraman Jeyakan...
materialswhenexcitedbyahigh-energyx-raysourceandisusedprimarilytodetermine amountsofparticularelementsinmaterials. Thiscourseisprimarilyconcernedwiththex-raycrystallographyofpowders.Incourse materialsyouwillcommonlyfindX-rayDiffraction,X-raypowderdiffraction,andthe ...
A Microfocus X-ray Tube Used with Focusing Collimators The design and performance are described of a low-power copper-target X-ray tube with an elongated focus foreshortened to a diameter between 10 and 20m. Th... UW Arndt,JVP Long,P Duncumb - 《Journal of Applied Crystallography》...
Owing to its unique penetrating power and high-resolution capability, X-ray imaging has been an irreplaceable tool since its discovery. Despite the significance, the resolution of X-ray imaging has largely been limited by the technical difficulties on X-
X-ray crystallography led to X-ray microscopes. Crystal structures direct and control X-rays much as lenses do with normal light energy. Using this principle, researchers were able to delve everdeeper into the structure of crystals. The fact that X-rays are absorbed by material led to ...
X-ray computed tomography (CT) is a commercially established modality for imaging large objects like passenger luggage. CT can provide the density and the effective atomic number, which is not always sufficient to identify threats like explosives and nar
We used two-dimensional 1H,15N HSQC chemical shift perturbation mapping of 15N-labeled Phe residues and x-ray crystallography to examine the ligand-dependent conformational dynamics of CYP119. Active site Phe residues were most affected by the binding of azole inhibitors and fatty acid substrates, ...
Free-electron lasers (FEL) are revolutionizing X-ray-based structural biology methods. While protein crystallography is already routinely performed at FELs, Small Angle X-ray Scattering (SAXS) studies of biological macromolecules are not as prevalent. SA