X-ray crystallography can be used to screen compounds that are not known ligands of a target biomolecule for their ability to bind the target biomolecule. The method includes obtaining a crystal of a target biomolecule; exposing the target biomolecule crytal to one or more test samples; and ...
As proteins are fundamental for Protein X-ray crystallography, HitGen's protein science team offers to design and generate high-quality proteins for advanced research applications. Single standard crystallographic services can also be provided, including initial crystallization, crystal optimization, data col...
X-ray Crystallography(X射线晶体衍射)IntroductiontoX-raycrystallography Why’sandHow’s WhydeterminestructuresWhyX-raysandcrystalsHowdowegetproteincrystalsHowdowecollectdiffractiondatafromacrystalHowdowedeterminethestructureHowcanweevaluatethequalityofourmodel Whydeterminestructures?BasicresearchoAnswersbasicquestions....
Recognizing that Bst DNA polymerase (DNAP) is functional in crystallo, we postulated that time-resolved X-ray crystallography could be used to determine the identity and order of intermediates in the catalytic cycle18. This method has been used previously to study the mechanism of DNA repair enzy...
Future directions and perspectives on the the role of crystallography as a tool to access the molecular mechanisms underlying enzymatic activity modulation upon treatment in pressurized fluids are also addressed. 展开 关键词: Enzyme Structure X-ray crystallography Pressurized fluids ...
In this chapter, types of crystallographic X-ray detectors in common use, and several under development, are listed and their salient features are summarized. Detector characteristics of particular importance to crystallography include the detective quantum efficiency, spatial resolution, stopping power, dy...
- 《Crystallography Reports》 被引量: 1发表: 2013年 Curved focusing crystals for hard X-ray astronomy A lens made by a properly arranged array of crystals can be used to focus x-rays of energy ranging from 30 to 500 keV for x-ray astronomy. Mosaic or curved... C Ferrari,E Buffagni,...
Neutron and X-ray scattering have proven to be two of the most powerful structural determination techniques. In particular, X-ray crystallography has been successfully applied to solving the crystal structures of proteins, beginning in the late 1950s with the structure of sperm whale myoglobin (Ken...
The incident beam is elastically scattered by the electrons of the target atoms into many specific directions which are characteristic of the atomic and molecular structure of the crystals, thus allowing a direct and quantitative access to the crystallography of the sample. In some beam-lines, the...
X-ray crystallography is an extremely precise means of imaging the exact structure of a given molecule or macromolecule in a...