steps. It is faster, stable, and more sensitive than MTT, XTT, MTS based assays. The assay correlates well with the [3H]-thymidine incorporation assay.The WST-1 assay protocol is very simple:- add the WST-1 assay reagent to the cell culture media and incubate for between 0.5 and 4 ...
MTT Cell Proliferation Assay Kit provides a convenient tool for studying the induction and inhibition of cell proliferation in any in vitro model. In this assay, MTT is taken up by cells due to its net positive charge and the plasma membrane potential. It is reduced to formazan by ...
WST-1 Assay Protocol for Cell Viability WST-1 assay protocol for cell viability and cytotoxicity measurements with preparation instructions, applications, FAQs, and troubleshooting.Technical Service Our team of scientists has experience in all areas of research including Life Science, Material Science, Ch...
ASSAY PROTOCOL Procedure 1. Seed cells in a 96-well plate at a density of 104-105 cells/well in 100 µl of culture medium with or without compounds to be tested. Culture the cells in a CO2 incubator at 37°C for 24-48 hours. 2. Add 10 µl of the WST-1 mixture to each ...
Product Information Sheet WST-1 Cell Proliferation and Cytotoxicity Assay Kit Catalog No.: AR1158 Introduction 1. WST-1 Cell Proliferation and Cytotoxicity Assay Kit is a sensitive and accurate assay for cell cytotoxicity and proliferation. The kit components are sufficient for performing up to 100 ...
One of the most convenient methods for measuring superoxide dismutase activity is a plate assay using xanthine oxidase and the water soluble tetrazolium WST-1. For reliable results with WST-1, certain aspects of the procedure need to be adhered to. This article describes an appropriate protocol ...