The WST-1 reagent offers a colorimetric assay to quantify cell proliferation, viability and cytotoxicity in a 96-well plate format without using radioactivity.
Detection PrincipleThe activity of SOD was measured by WST-1 method in this kit and the principles of the WST-1 is as follows. Xanthine Oxidase (XO) can catalyze WST-1 react with O2.- to generate a water-soluble formazan dye. SOD can catalyze the disproportionation of superoxide ani...
Detection Principle Detect total content of NAD+ and NADH: Ethanol generates acetaldehyde under the action of enzyme. Meanwhile, NAD+ is reduced to NADH, NADH, under the action of hydrogen transmitter, transfer electrons to WST-8 to produce the yellow product, which has a characteristic absorptio...