Reverse-phase (RP) HPLC separates oligos based on their hydrophobicity, while ion-exchange (IE) HPLC separates oligonucleotides based on their charge. IDT uses HPLC for modified oligos that include additions such as linkers, spacers, non-standard bases, and hydrophobic modifications. The type of ...
Intensive properties given for a liquid compound, Why is it necessary to determine more than one property to identify your unknown? Describe the differences between normal phase and reverse phase chromatography. Describe the separation process that resulted in the...
The selection rules are the sets of changes occurring in the quantum numbers due to the transition of one or more electrons from one state to another. The transition probability of electrons can never be zero. Answer and Explanation:1 As with rotational and vibrational trans...
if the toxic load is too heavy, it can cause overactivity of Phase I enzymes, which can have the reverse effect of converting relatively harmless substances into potential DNA-damaging carcinogens
Plasma zinc concentration was measured using flame atomic absorption spectrophotometry (Shimadzu AA-6501S, Kyoto, Japan) [16]. Plasma retinol concentration was measured by reverse phase HPLC using C18 column (Discovery C18, 25cmX4mm, 5µm, Cat# 504971) and detected at 325nm [17]. Plasma fer...