Reverse-phase (RP) HPLC separates oligos based on their hydrophobicity, while ion-exchange (IE) HPLC separates oligonucleotides based on their charge. IDT uses HPLC for modified oligos that include additions such as linkers, spacers, non-standard bases, and hydrophobic modifications. The type of ...
ChulaCov19 mRNA vaccine 2 Day 0 + 21 N/A IM Chulalongkorn University N/A Phase 1 NCT04566276 N/A Note: IM, intramuscular; N/A, not apply; ND, not done.One superior advantage of mRNA vaccines is their use of the cellular translational machinery and other cytosolic components in producing...
Plasma zinc concentration was measured using flame atomic absorption spectrophotometry (Shimadzu AA-6501S, Kyoto, Japan) [16]. Plasma retinol concentration was measured by reverse phase HPLC using C18 column (Discovery C18, 25cmX4mm, 5µm, Cat# 504971) and detected at 325nm [17]. Plasma fer...
Furthermore, there is always extra time spent on mobile phase elution between each measurement for the purposes of removing residual impurities from the previous sample as well as equilibrating the column before the analysis of a new sample. This long analysis results in an important delay between...