The principle depends on HPLC separation of the two drugs on the Phenomenex Column (150mm×4.6mm, 5μm) with a mobile phase containing Methanol: Ortho phosphoric acid (55: 45, v/v) and the flow rate being 1.0 mL/min. UV detection at 270 nm with a retention ...
Non-labelled oligonucle- otides were Reverse Phase Cartridge•Gold purified; the double-dye labelled FAM-HpE6-Dabcyl was Reverse Phase HPLC purified. Oligonucleotides were dissolved in bi-distilled water (at a concentration of 200 μM) and stored at −20 °C. ...
Results and Discussion RP-HPLC-MALS technique. The principle of RP-HPLC-MALS is the combination of RP chromatography with an online MALS detector. As shown in Fig. 1, multiple hydrophobic areas of protein molecules interact with the alkyl silane-derivated surface of the stationary phase. ...
Immunological Activity and Residual AOT in the Aqueous Phase Although it would be difficult to quantitate the specific antigen-antibody reaction, the antiserum was used for double gel diffusion assay based on the principle that both reactants (antigen and antibody) move through an inert media and ...
1.Principle of nanometer catalyst preparation through reverse microemulsion method and its application in preparation of high temperature combustion catalyst were introduced.综述了反相微乳法制备纳米催化剂的原理及其在制备高温燃烧催化剂上的应用。 英文短句/例句 1.Study on the preparation of corn starch micros...
Test articles were separated on a C18 column using a gradient elution with an acetonitrile/water mixture containing 0.1% formic acid as the mobile phase. Chromatographic conditions and MS parameters were optimized for the tested compound before sample analysis. Concentration...
The HPLC system uses an ODS2 reverse phase column (5μm particle size) eluted with a methanol, acetonitrile and water gradient with detection at 280nm. In addition to providing baseline resolution of most components, the method employs a spectrometric detector which enables the precise ...
On reverse phase HPLC, CDF is poorly fra ctiona ted . and shows a high polarily. With molecular seave chromatography by HPLC on Protein I 125P column, CDF is purified 10 times over original RAF dry weight. The apparent molecular weight of CDF is 13, 000 daltons. When r a t amnio...
Test articles were separated on a C18 column using a gradient elution with an acetonitrile/water mixture containing 0.1% formic acid as the mobile phase. Chromatographic conditions and MS parameters were optimized for the tested compound before sample analysis. Concentrations of LDC4857 were calculated...
Upon reaction completion, the mixture was filtered and purified by reverse phase HPLC (ACN/water with 0.1% TFA modifier) to afford (3-((5-(3-chloro-5-cyanophenoxy)-6-oxo-4-(trifluoromethyl)pyrimidin-1(6H)-yl)methyl)-5-(4-fluorophenyl)-6-oxopyridazin-1(6H)-yl)methyl dihydrogen ...