1. 基因组随机扩增 单个细胞全... ... ) genome amplification 基因组扩增 )whole-genome amplification基因组随机扩增) cell expansion 细胞扩增 ... www.dictall.com|基于2个网页 2. 全基因体放大 使用单细胞排序法,连同全基因体放大(whole-genome amplification)及下一代的排序法,该团队能证实,事实上存在三...
The article presents information on the use of the REPLI-g kits in whole genome amplification. The kits provide highly uniform DNA amplification across the entire genome, with minimal sequence bias. Various samples can be used, including genomic DNA, fresh or dried blood, buffy coat, and tissue...
Amplification fromsingle cellsorlow cellnumbers Copynumber analysis Stabilityof amplifiedDNA Cleanup recommendatio ns REPLI-gFFPEprinciple DNAfromparaffin-embeddedtissuesamplesusuallyshows highdegradationduetothefixationprocedure(see “Workingwithfixedtissuesamples”).Asaresult,the DNAobtainedfromFFPEtissuesamplesisnot...
whole genome amplification (WGAlow copy number (LCNforensic scienceprimer extension preamplification (PEPdegenerate oligonucleotide primed PCR (DOPmultiple displacement amplification (MDAMethods and Protocolsdoi:10.1007/978-1-4939-2990-0Scion Publishing Ltd...
产品名称:Whole Genome Amplification Kit 描述: 全基因组扩增试剂盒基于多重链置换扩增的Phi29 DNA聚合酶等温扩增体系,Phi29 DNA聚合酶是从Bacillus subtilis噬菌体Phi29中克隆出的嗜温DNA聚合酶,利用基因重组技术,在大肠杆菌中进行表达后经多次纯化分离而得。Phi29 DNA聚合酶具有很强的链置换活性,可实现对DNA复杂结...
海外直订Whole Genome Amplification 作者:No出版社:Scion Publishing Ltd出版时间:2005年08月 手机专享价 ¥ 当当价 降价通知 ¥895.00 配送至 广东佛山市 至 北京市东城区 服务 由“中华商务进口图书旗舰店”发货,并提供售后服务。 加入购物车 中华商务进口图书旗舰店 明星店铺 进入店铺 收藏店铺 ...
As a WGA method, ligation-mediated PCR (LM-PCR) has the shortcoming that strict conditions are required to avoid biased DNA amplification. Here, we used TspR I endonuclease to digest the target DNA into fragments with 9-nt-long (NNCASTGNN) sticky ends in which 4-5 different bases from ...
Sequencing of a single-cell genome requires DNA amplification, a process prone to introducing bias and errors into the amplified genome. Here we introduce a novel multiple displacement amplification (MDA) method based on the unique DNA primase features o
haematobium using an established protocol34, and 200 ng was subjected to whole-genome amplification (WGA) using the REPLI-g Midi Kit (Qiagen). Total DNA amounts were determined using a Qubit fluorometer dsDNA HS Kit (Invitrogen), and DNA integrity was verified by agarose gel electrophoresis. ...
Multiple displacement amplification from single bacterial cells Multiple Displacement Amplification from single bacterial cells. In: Hughes S, Lasken R, eds. Whole Genome Amplification. Banbury: Scion Publishing Ltd., ... R Lasken,A Raghunathan,T Kvist,... - Whole Genome Amplification 被引量: 20...