Comparison of whole genome amplification methods for further quantitative analysis with microarray-based comparative genomic hybridization. Taiwan J Obstet Gynecol 2008;47:32-41.Lee YS, Tsai CN, Tsai CL, et al. Comparison of whole genome amplification methods for further quantitative analysis with ...
Whole genome amplificationTspR I endonucleaseDNA sequencing, genotyping, detection, and SNPs analysis usually require large amount of DNA. In the case that only tiny amount of DNA can be collected from samples, the whole genome amplification (WGA) technique is a good choice for its high ...
Whole genome amplification (WGA) has become an invaluable tool to perform copy number variation (CNV) detection in single, or a limited number of cells. Unfortunately, current WGA methods introduce representation bias that limits the detection of small CNVs. New WGA methods have been introduced tha...
whole genome amplification (WGAlow copy number (LCNforensic scienceprimer extension preamplification (PEPdegenerate oligonucleotide primed PCR (DOPmultiple displacement amplification (MDAMethods and Protocolsdoi:10.1007/978-1-4939-2990-0Scion Publishing Ltd...
low nucleotide error rates due to the high fidelity of the polymerase, produce very long amplification products and cover the genome more completely. Problems that affect all amplification methods to some degree are chimera formation and preferential amplification of one allele (allelic dropout, ADO)....
P20: Improving Whole Genome Amplification methods forpre-implantation diagnosisPublication » Improving whole genome amplification methods for preimplantation diagnosis.doi:10.1016/j.ejmg.2005.10.032elsevierEuropean Journal of Medical Genetics
100timeslowerthanTaqDNApolymerase-basedmethods(see PCR-basedWGA). SchematicrepresentationofREPLI-gDNAamplification. Phi29DNApolymerasemovesalongtheDNAtemplatestrand displacingthecomplementarystrand.Thedisplacedstrand becomesatemplateforreplicationallowinghighyieldsof high-molecular-weightDNAtobegenerated. REPLI-gprocedure...
GenomePlex is a Whole Genome Amplification (WGA) method that allows the researcher to generate a representative, ~500-fold amplification of genomic DNA. The kit utilizes a proprietary amplification technology based upon random fragmentation of genomic DNA and conversion of the resulting small fragments ...
We present a survey of single-cell whole-genome amplification (WGA) methods, including degenerate oligonucleotide-primed polymerase chain reaction (DOP-PCR), multiple displacement amplification (MDA), and multiple annealing and looping-based amplification cycles (MALBAC). The key parameters to characterize...
PCR-mediated whole genome amplification of phytoplasmas A method was developed for genome analysis of phytoplasmas, bacterial plant pathogens that cannot be cultivated in vitro in cell-free media. The procedure ... M Garcia-Chapa,A Batlle,D Rekab,... - 《Journal of Microbiological Methods》 被引...