Whole exome sequencing is a powerful tool to investigate genetic variations underlying cancers, Mendelian diseases, and complex human disorders.
Whole Exome Sequencing (WES) is a groundbreaking genetic test that examines the coding regions of your DNA. These regions, known as exons, contain crucial information about the proteins responsible for various biological functions. Whole Genome Sequencing (WGS) has also revolutionized genomics research...
Learn about what whole exome sequencing is, why it’s important, and how it’s furthering discoveries in cancer research.
About Whole Exome Sequencing The exome, comprising about 30 million base pairs and representing 22,000 genes, makes up roughly 2% of the genome. It is estimated that approximately 85% of disease-causing variants can be found within these protein-coding regions. WES therefore allows for a ...
Learn about whole exome sequencing (WES), a targeted next generation sequencing method that identifies all the protein-coding genes (exons) in the genome.
全外显子测序(Whole Exome Sequencing,WES) 标准分析流程 全外显子测序的标准分析流程通常包括以下步骤: 质量控制(Quality Control,QC) 对原始测序数据进行质量控制,包括去除低质量的序列片段、过滤掉低质量的碱基和低覆盖度的测序片段等。 读取比对(Read Alignment) 将过滤后的测序数据比对到参考基因组上,以确定每...
全外显子测序(Whole Exome Sequencing, WES):通过目标区域获取技术对外显子区域的DNA进行测序,可获取基因编码区域的核酸突变信息,从而研究其与疾病和其它生物性状相关性。相对于全基因组测序,全外显子可显著降低成本。 技术流程 技术参数 测序平台与方式:HiSeq, PE150捕获平台:Agilent SureSelect测序深度:100X,200X...
全基因组测序,顾名思义,即是对生物体整个基因组序列进行测序,可以获得完整的基因组信息。而全外显子测序(Whole Exome Sequencing, WES)仅是对外显子区域的测序。相比全基因组测序,外显子测序更为简便,测序成本相比也会更低,测序后数据的分析也更为简单。另外如果仅关注已知的基因,可以更依赖于靶向测序。
Whole-exome sequencing (WES) is application of the next-generation technology to determine the variations of all coding regions, or exons, of known genes. WES provides coverage of more than 95% of the exons, which contains 85% of disease-causing mutations in Mendelian disorders and many ...
What Is Whole Exome Sequencing, WES? WES is a cost effective alternative to Whole Genome Sequencing, WGS. The human exome consists of approximately 180,000 exons, which constitutes about 1-2% of the human genome. These exon protein coding regions harbor variants which are much more likely to...