Exome sequencing is a fast and affordable alternative to using whole genome sequencing for your research, allowing you to identify somatic and germline genetic variants within coding regions of genes without the additional expense and data analysis challenges associated with whole genome ...
Whole Exome Sequencing Workflow 1Construct Library Ion AmpliSeq Exome RDY Kit Hands-on time: ~50 minutes 2Prepare Template Ion Chef System Hands-on time for Ion Chef System: ~15 minutes 3Run Sequence Ion GeneStudio S5 Plus or Ion GeneStudio S5 Prime Syst...
The sheer volume of data generated from whole exome sequencing and whole genome sequencing presents a challenge for many researchers faced with the prospect of assembling and analyzing it. Whether you are working with whole genome, whole exome, or other targeted sequencing data, the resulting ...
Whole exome sequencing of 251 individuals from 97 NPC families led to the identification of numerous rare variants possibly linked to NPC, including variants within genes involved in Notch signaling (NOTCH1, DLL3), magnesium transport (NIPAL1), EBV entry into epithelial cells (ITGB6), modulation...
ARTICLE Received 1 Aug 2014 | Accepted 20 Feb 2015 | Published 15 Apr 2015 DOI: 10.1038/ncomms7687 Whole-exome sequencing implicates UBE3D in age-related macular degeneration in East Asian populations Lv-Zhen Huang1,2,3,*, Ying-Jie Li4,*, Xue-Feng Xie5,*, Jing-Jing Zhang1,2,3,*,...
Whole exome sequencing workflows usingSNP & Variation Suite(SVS) was presented in a recentwebcast, by Dr. Robert Hamilton from the Hospital for Sick Kids. In particular, he performed some filtering on his data to look for only heterozygous variants in his sample of interest, removed variants ...
Whole exome sequencing of a Saudi family and systems biology analysis identifies CPED1 as a putative causative gene to Celiac Disease Author links open overlay panelHifaa A. Bokhari a 1, Noor Ahmad Shaik a b 1, Babajan Banaganapalli a b 1, Khalidah Khalid Nasser b g, Hossain Ibrahim ...
Flowchart of WES (Whole Exome Sequencing) analysis: T1, T2, T3 design the three nuclear families respectively, p (paternal) for the father, m (maternal) for the maternal and c for the affected child. The number in dark boxes indicate the total number of variants identified respectively in ...
Whole exome FFPE sample library preparation and sequencing For the FFPE study, SureSelect (Agilent) WES libraries were prepared according to the manufacturer’s instructions for 200 ng of DNA input, including reducing the shearing time to four minutes. Additionally, the adaptor-ligated libraries ...
Several large sequencing projects are ongoing such as the 1000 Genomes project4, the Exome sequencing project (ESP)5,6 and the GoNL project7. We have sequenced the whole genomes of 2,636 Icelanders using Illumina technology. The individuals were selected for sequencing based on having a wide ...