In line with this, we observed that paclitaxel induced changes to malignant epithelial cells that suggest an altered capability to interact with immune cells via secretion of CXCL1 and CXCL8. Tumor-derived CXCL1 can recruit immunosuppressive myeloid cells that in turn inhibit CD8+ T cell ...
The stimulatory and inhibitory effects of somatostatin upon PTPase and Raf-1 activities, respectively, in S49 cells are likely to be reflective of important physiologic functions of somatostatin in immune cells. Identification of the G-protein regulated PTPase(s) and evaluation of its significance ...
In a pathological context, exosomes have been mainly studied in cancer, as they regulate the immune response against cancer cells, and promote metastasis [10, 35]. How- ever, growing evidence suggests that exosomes may also be involved in the induction and spreading of pathology in a range of...
To generate a monolayer culture, MIOs were cultured for at least 1 passage with Intesticult OGM Human medium. Before seeding cells for monolayer, Transwell inserts (6.5 mm, 0.4 μm pore size, polyester, Corning Inc.) were coated with diluted Matrigel (1:50 in PBS) for 3–4 h....
immune cells in the mouse brain under homeostasis and analyzed the expression ofCasp4expression levels in this scRNA-seq atlas [32]. In agreement with our findings, we found thatCasp4is mainly/highly expressed by microglia which also highly express CX3CR1 (Supplementary Fig.1). Therefore, we ...
Silencing expression of ribosomal protein L26 and L29 by RNA interfering inhibits proliferation of human pancreatic cancer PANC-1 cells. Mol Cell Biochem. 2012;370(1–2):127–39. Li XC, Hu QK, Chen L, Liu SY, Su S, Tao H, et al. HSPB8 promotes the fusion of autophagosome and ...
CHO-Lec2 cells were transduced with a lentiviral vector encoding the full-length human Gb3/CD77 synthase (CHO-Lec2 A4GALT) or its p.Q211E mutein (CHO-Lec2 A4GALT Q211E). The anti-P1 antibody (P3NIL100) detected Galα1→4Galβ1→4GlcNAc structures on several glycoproteins in CHO-Le...
Such treatment only slightly reduced c-FLIPL; however, c-FLIPS became almost unde- tectable, suggesting that c-FLIPS rather than c-FLIPL, confers resistance of T cells to CD95-mediated death in the context of immune responses.25 Stimulation of TCR/CD3 in human peripheral blood T lymphocytes...
Within 60 min following a single injection of GMI-1271, the number of human CD138+ cells in the PB increased, and persisted increasing for at least 24 h (2.37 vs 0.03%, P o0.001, Figure 4). This effect was consistent with GMI-1271 disrupting the tumor microenvironment and mobilizing MM...
These neuronal progenitor cells (termed NPCs below) are found in the central nervous system. They also express Nestin and are able to differentiate into neurones, astrocytes and oligodendrocytes. Neuronal progenitor cells are CD133-positive; this cell surface marker was originally found on haematopoiet...