Equal amounts of SILAC lysates were then mixed 1:1, reduced with DTT, and alkylated with CAA before being resolved on SDS-PAGE (8–12 %w/v, Invitrogen). Separated proteins were fixed in the gel and visualized with colloidal Coomassie staining (Invitrogen). Each gel lane was excised and ...
“Cadherin switch” and decreased the expression level of TCF8/ZEB1, β-Catenin, and Vimentin. Mechanistically, EGCG inhibited the Akt pathway in a time-dependent manner, by suppressing IGFR phosphorylation and inducing Akt degradation. Co-treatment with catalase or N-Acetyl-L-cysteine did not ...
After incubation, five fields in each well were randomly selected for counting the fused and unfused cells under an inverted fluorescence microscope (Invitrogen, Waltham, MA, USA). The percentage of cell–cell fusion ((number of fused cells/number of fused and unfused cells) × 100%) was ...