germGerm 系统标签: germexpressionwheatwgcfnesgprovide EngineeringofawheatgermexpressionsystemtoprovidecompatibilitywithahighthroughputpET-basedcloningplatformLiZhao•KateQ.Zhao•RobinHurst•MichaelR.Slater•ThomasB.Acton•G.V.T.Swapna•RituShastry•GregoryJ.Kornhaber•GaetanoT.MontelioneReceived:15...
2010. The wheat-germ cell-free expression system. Curr Pharmaceut Biotechnol 11:272-278.Takai, K., Sawasaki, T., and Endo, Y. (2010) The wheat germ cell-free expression system. Curr. Pharm. Biotechnol. 11, 272-278Takai, K., Sawasaki, T., and Endo, Y. (2010) The wheat-germ ...
Advantages of the wheat germ cell-free extract expression system: Save time and increase overall yield of functional, soluble, full-length proteins; Suitable for the expression of toxic proteins such as kinases; Can be labeled with modified tRNA; ...
Wheat germ cell-free methods provide an important approach for the production of eukaryotic proteins. We have developed a protein expression vector for the TNT SP6 High-Yield Wheat Germ Cell-Free (TNT WGCF) expression system (Promega) that is also compatible with our T7-based Escherichia coli ...
1708Vol.76,No.40019-9567/08/$08.00 0doi:10.1128/IAI.01539-07Copyright©2008,AmericanSocietyforMicrobiology.AllRightsReserved.WheatGermCell-FreeSystem-BasedProductionofMalariaProteinsforDiscoveryofNovelVaccineCandidates †TakafumiTsuboi,1,2*SatoruTakeo,1HideyukiIriko,2‡LingJin,2MasateruTsuchimochi,1...
Fig. 1. Schematic illustration of wheat germ cell free protein expression system and its applications. A.The DNA template for protein expression is prepared by cloning into pEU expression vectors or by direct PCR amplification followed by in vitro transcription to get mRNA. The mRNA is then mixed...
For more information, see the Protein Expression chapter of the Protocols & Applications Guide. 注意事项 Each system contains 2 × 200μl of both Rabbit Reticulocyte Lysate Wheat Germ Extract; this is sufficient for 12 reactions using the Rabbit Reticulocyte Lysate 12 reactions using the Wheat Germ...
英文名称: ERK8 Lysate (CFS wheat germ cell free in vitro expression system), His tagged 总访问: 563 国产/进口: 进口 半年访问: 16 产地/品牌: Abnova 产品类别: 抗体 规格: 100 ul 最后更新: 2024-10-11 货号: CAS 号: 参考报价: 3000元 立即询价 电话咨询 ...
Wheat germ significantly improved the enzyme activity of the female broodstock (P<0.05). The activity of superoxide dismutase (SOD), catalase (CAT), and total antioxidant capacity (T-AOC) enzyme was the highest in the 10% wheat germ addition diet, with which the content of malondialdehyde (...
摘要: We have rationally constructed a novel regulation-type of artificial riboswitch that ligand-dose dependently upregulates translation initiation mediated by a 3' cap-independent translation element (3' CITE) with no major hybridization switches in a plant expression system (wheat germ extract). ...