N. (2006) Wheat germ cell-free expression system for protein production. Curr. Protoc. Protein Sci. Chapter 5:Unit 5 18.Vinarov DA, Newman CLL, Tyler EM, Markley JL, Shahan MN (2006) Wheat germ cell-free expression system for protein production. Curr Protoc protein Sci Editor board John...
Engineeringofawheatgermexpressionsystemtoprovide compatibilitywithahighthroughputpET-basedcloningplatform LiZhao • KateQ.Zhao • RobinHurst • MichaelR.Slater • ThomasB.Acton • G.V.T.Swapna • RituShastry • GregoryJ.Kornhaber • GaetanoT.Montelione Received:15December2009/Accepted:11Ju...
Wheat germ cell-free methods provide an important approach for the production of eukaryotic proteins. We have developed a protein expression vector for the TNT SP6 High-Yield Wheat Germ Cell-Free (TNT WGCF) expression system (Promega) that is also compatible with our T7-based Escherichia coli ...
Results A 70 000 Mr, soluble extracellular domain fragment of the PbTIP which consistent with expectation was obtained by the wheat germ expression system, the concentration of target protein was about 0.1 mg / ml. Conclusion The soluble extracellular domain fragment of the PbTIP can be ...
Expression SystemWheat germ Protein FamilyLectins Protein FormHeterodimer FormLyophilized Protein SubtypeAgglutinin Purity or Quality GradeSee Certificate of Analysis ProteinFluorescent lectins Ligand TypeN-acetylglucosamine and N-acetylneuraminic acid (sialic acid) residues Unit Size5 mg Contents & Storage Stor...
Common NameWheat Germ Agglutinin, Texas Red™-X Conjugate ConjugateTexas Red-X SpeciesWheat RecombinantNative Protein TagNone Shipping ConditionRoom Temperature Expression SystemWheat germ Protein FamilyLectins Protein FormHeterodimer FormLyophilized Protein SubtypeAgglutinin Product LineTexas Red™ Purity or...
expression.Here,wereporttheapplicationofthewheatgermcell-freesystemforthesuccessfulproduc- tionofmalariaproteins.Forproofofprinciple,thePfs25,PfCSP,andPfAMA1proteinswerechosen. ThesegenescontainveryhighA/Tsequencesandarealsodifficulttoexpressasrecombinantproteins.In ...
For more information, see theProtein Expressionchapter of the Protocols & Applications Guide. 注意事项 Each system contains 2 × 200μl of both Rabbit Reticulocyte Lysate Wheat Germ Extract; this is sufficient for 12 reactions using the Rabbit Reticulocyte Lysate 12 reactions using the Wheat Germ ...
, catalase (CAT), 14-3-3, glutathione S-transferase (GST), PGK, and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) from the wheat germ protein expression system (cell free) were expressed as fusions with the His-tag and/or the Halo-tag and showed clear Khib signals; the positive control...
英文名称: CDK4 Lysate (CFS wheat germ cell free in vitro expression system), His tagged 总访问: 656 国产/进口: 进口 半年访问: 30 产地/品牌: Abnova 产品类别: 抗体 规格: 100 ul 最后更新: 2024-12-5 货号: CAS 号: 参考报价: 3000元 立即询价 电话咨询 ...