2010. The wheat-germ cell-free expression system. Curr Pharmaceut Biotechnol 11:272-278.Takai, K., Sawasaki, T., and Endo, Y. (2010) The wheat germ cell-free expression system. Curr. Pharm. Biotechnol. 11, 272-278Takai, K., Sawasaki, T., and Endo, Y. (2010) The wheat-germ ...
Advantages of the wheat germ cell-free extract expression system: Save time and increase overall yield of functional, soluble, full-length proteins; Suitable for the expression of toxic proteins such as kinases; Can be labeled with modified tRNA; ...
Wheat Germ Cell-Free System-Based:小麦胚芽无细胞系统细胞,系统,wheat,germ,小麦胚芽,WHEAT,GERM,Wheat,Germ,based 文档格式: .pdf 文档大小: 282.62K 文档页数: 8页 顶/踩数: 0/0 收藏人数: 1 评论次数: 0 文档热度: 文档分类: 论文--毕业论文 ...
Fig. 1. Schematic illustration of wheat germ cell free protein expression system and its applications. A.The DNA template for protein expression is prepared by cloning into pEU expression vectors or by direct PCR amplification followed by in vitro transcription to get mRNA. The mRNA is then mixed...
英文名称: ERK8 Lysate (CFS wheat germ cell free in vitro expression system), His tagged 总访问: 563 国产/进口: 进口 半年访问: 16 产地/品牌: Abnova 产品类别: 抗体 规格: 100 ul 最后更新: 2024-10-11 货号: CAS 号: 参考报价: 3000元 立即询价 电话咨询 ...
摘要: We have rationally constructed a novel regulation-type of artificial riboswitch that ligand-dose dependently upregulates translation initiation mediated by a 3' cap-independent translation element (3' CITE) with no major hybridization switches in a plant expression system (wheat germ extract). ...
Recently, some groups have reported on cell-free synthesis of functional membrane proteins (MPs) in the presence of exogenous liposomes (liposomes). Previously, we reported synthesis of a functional AtPPT1 plant phosphate transporter that was associated
Wheat germ cell-free methods provide an important approach for the production of eukaryotic proteins. We have developed a protein expression vector for the TNT SP6 High-Yield Wheat Germ Cell-Free (TNT WGCF) expression system (Promega) that is also compatible with our T7-based Escherichia coli ...
产品名称:RFK Lysate (CFS wheat germ cell free in vitro expression system), His tagged .0订购此产品 供应商:Abnova 规格:100 ul 目录价:￥:3000 库存状态: CAS编号: 应用范围: 种属来源: 相关信息: 保存条件: 说明书地址:点击查看详细 打印此页关闭此页 ...
However, the system requires specialized techniques to maintain high infectivity of virus and degradation of the expressed protein by intrinsic proteases needs to be prevented because baculovirus infection induces cell death. Compared to in vivo expression systems, wheat germ cell-free translation systems...