each unique DNA molecule in the library is bound to the surface of a bead or a flow-cell and PCR amplified to create a set of identical clones. In the case of Ion Torrent technology, a process called “templating” is used to ...
What is the role of DNA gyrase? What are nucleosomes? a.One phosphate group, deoxyribose sugar, and a base b.One phosphate group, a ribose sugar, and a base c.Dense regions of DNA that stain darkly d."Beads" formed by DNA wrapped around histones What is the role of the polymerase ...
Why is cDNA used as a template in RT-PCR, not mRNA? What is the benefit of RNA being single-stranded? How do RNA and magnetic beads bind together when performing RNA extraction? What are the 3 types of RNA and what are their functi...
Although they showed that PCR has a higher sensitivity than standard microscopy for ectoparasite detection, they concluded that PCR on swabs is less accurate [190]. For reptiles, the detection of mercury in skin scutes has been described [191]. It would be of interest to assess whether the ...
in which there is a direct connection between the genetic activity, protein activity and the metabolic activity itself” [15], while metabonomics is “the quantitative measurement of the multivariate metabolic responses of multicellular systems to pathophysiological stimuli or genetic modification” [15,16...
Instruments include traditional and real-time thermocyclers while reagents and consumables include enzymes, dNTP's, template DNA, primers and probes, buffers, master mixes, nuclease free water and others (tracking dye and wax beads). IQ4I Research & Consultancy Published a New Report on "PCR Te...
The liver is essential for processes such as metabolism, drug detoxification and plasma protein production. The two major parenchymal cell types in the liver are hepatocytes and biliary epithelial cells (BECs; cholangiocytes). Hepatocytes perform most of the liver functions, while bile ducts formed...
Schwan and others have demonstrated that the binding of the FimH adhesin leads to a positive feedback loop by comparing fim gene expression in the presence of mannose-coated Sepharose beads and normal Sepharose beads. More specifically, when analyzing transcripts, in the presence of mannose-coated ...
Briefly, 100 mg of rat fecal pellet was homogenized in the AquaStool solution with 0.1 mm beads at 0.6 m/s for 40 s. AquaRemove was added to remove potential PCR inhibitors per manufacturer’s instruction followed by ethanol/NaCl precipitation for further purification. DNA Samples were sent ...
As for personal care products, only Disodium EDTA is detected in wastewater, while in groundwater the concentrations of all the analyzed substances are lower than the instrumental detection limit. Keywords: sewer; leakage; urban semi-confined groundwater; personal care products; microbiological ...