Why is PCR used in the process of DNA sequencing? Why is Taq DNA polymerase used for PCR? Why are primers needed for DNA replication? Why is DNA polymerase used in PCR? Why is there no helicase in a PCR? Why do you need a forward and reverse primer in PCR?
Why is DNA double stranded and RNA isn't? Why is PCR used in the process of DNA sequencing? Why are nucleic acids important? Why does DNA contain thymine instead of uracil? Why is cytosine used in DNA instead of uracil? Why are dideoxynucleotides used in DNA sequencing?
What is the difference between PCR and nested PCR when we should use nested PCR? Conventional PCR (C-PCR) has been used to detect specific target genes in various microorganisms (5, 6, 13). Nested PCR (N-PCR) wasdeveloped to improve sensitivitybut can give erroneous positive results due t...
2. The wrong PCR conditions used You will be surprised how common this problem is and how easy it is to make. You may have incorrectly programmed in the wrong cycle conditions recommended for your PCR reaction. Sometimes if multiple users are using the same PCR machine, it is possible someo...
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Why is Southern blotting better than PCR? Whereas Southern blotting is labor intensive and requires a large amount of high-quality DNA, real-time PCR has several advantages includingeasier automation, higher throughput screening, and a lower requirement for the amount of DNA used saving the research...
acDNA Amplified Fragment Length Polymorphism (cDNA-AFLP) and Relative Quantification (RQ-PCR) were used to study gene expression of P. trifoliata in response to 10, 24 and 55 h of low temperature (4_C) treatment and a gradual 3 weeks long cold-acclimation regime. 正在翻译,请等待...[trans...
Why is there a need for the primase to make an RNA primer for DNA replication? How are RNA genomes replicated? What is the reason/advantage of storing genetic information in DNA, but not in RNA? Why are RNA primers used in replication whereas DNA primers are used in PCR?