an identical copies of the DNA. The enzymes must work in sequence for the reaction to occur. One of the enzymes in the replication of DNA is the DNA polymerase. This is the heart of the replication process of the DNA. The synthesis of a new strand is in the direction of 5' to 3'...
What is the purpose of DNA polymerase? How is PCR used in ribosomal DNA analysis? Can gel electrophoresis be used for genetic testing? How does genomics rely on DNA sequencing? How does DNA ligase work? How reliable is gel electrophoresis?
The aim of our laboratory is to determine the mechanisms of resistance to radiotherapy and chemotherapy in cancer cells. We recently discovered that when the expression of a gene called DNA Polymerase theta (POLQ) is reduced using siRNA, cancer cells become more sensitive to radiotherapy 1. The ...
The first step in a PCR cycle is to heat the sample to 95°C. What is the purpose of heating the sample?A.To separate the two strands of DNAB.To allow the primer to anneal to the templateC.To produce the optimal temperature for Taq dna polymerase to workD.To produce the optimal ...
10.in which process,transcription or translation,do each of the following function:RNA polymerase,ribosomes,nucleotides,tRNA,pre-mRNA,DNA A site,anticodon,amino acids? 相关知识点: 试题来源: 解析 最上面的算是第二问吧?2、如楼上所说,一是因为DNA不能通过核孔,二是DNA为双螺旋结构,转录成RNA翻译更...
What does PCR stand for? What is the purpose of perform PCR reaction? Why did you need to perform the DNA extraction technique(Part I of this lab activity)prior to performing the PCR technique? What is the first substance that you added to...
PCR is a molecular technique frequently applied to amplify regions of interest in DNA, resulting in millions or billions of copies. The PCR reaction is made possible through the use of the thermostable DNA polymerase enzyme known as Taq polymerase. Taq refers to the heat-resistant bacteria (Therm...
DNA polymerase is an enzyme responsible for synthesizing new strands of DNA during DNA replication. It adds nucleotides to the growing DNA strand, using a DNA template and a primer as a starting point. DNA polymerase specifically catalyzes the formation of phosphodiester bonds between the added nucl...
PCR is a Polymerase Chain Reaction. Kary Mullis developed it in 1983. It is a technique used to amplify a specific segment of DNA. The technique mainly involves three steps: denaturation, annealing, and elongation. This technique is commonly used in molecular biolog...
These clinical samples are difficult to collect and generally only contain a small amount of usable mRNA. In a singleplex relative quantification experiment, only one gene—either the gene of interest or the control—is amplified in each well. Assuming that each assay...