Retention time of each peak is marked above the peak and in the tabulated data below the chromatogram details of the retention time, area (as digital units), peak area%, height and height %. You can observe that due to non-ideal shape of peaks percentage area is different from percentage...
Chromatography Type:Liquid Chromatography; Methyl Alcohol Flow Rate:1ml/Min; Detection Wavelength:254nm; Sample:Naphthalene(1*10-7g/Ml); Peak Area of Fluorene:Rsd=0.048%; Retention Time of Fluorene:Rsd=0.017%; After-sales Service:Free Spare Parts; Online Technical Support; ...
Split, splitless, and on-column are the most widely used sample-introduction modes in capillary gas chromatography. Under ideal conditions, solutes are eluted to the detector as short discrete bands, yielding sharp narrow peaks. Problems occasionally are encountered with sample introduction into the ...
What would be the effect on a chromatographic peak of introducing the sample at too slow a rate? What is the definition of retention time? Describe the factors affecting the rate of evaporation. Describe the effect of pressure on supercritical fluid chrom...
Response Factor = Peak Area / Concentration It is important to remember that variations in a gas chromatography (GC) system and analysis methodology can be the cause of a deviation in the response factor. Quantitative Analysis Quantitative analysis is the determination of a compound’s concentration...
Data integrity is an increasing area of concern for laboratories. In many regulated industries laboratories must demonstrate and document the suitability of analytical instruments and software for their intended use. This focus on compliance extends to how the instrument and software performance is ...
What is the difference between a line spectrum and a continuous spectrum? What is meant by a phase transition? What are the possible phase transitions that can occur? What are the differences between band broadening and peak in chromatography? If your test showed the lower layer to be t...
This entirely experimental area of chemical dynamics is known as kinetics. Reaction rates, as they are called, vary immensly: some reactions are completed in microseconds, others may take years; many are so slow that their rates are essentially zero. To make things even more interesting, there...
the folic acid and its derivatives in plant leaves are detected. The derivatives were quantitatively measured, and the peak area of the main peak was recorded. The peak area was automatically calculated according to the external standard method to obtain tetrahydrofolate, 5-methyl-tetrahydro...
Each peak area was transformed using a Hellinger transformation adequate for matrices with many zeros [70], and afterwards Pareto scaling was applied, which is useful for chromatographic analysis on a GC-MS platform [71]. In order to investigate the hypothesis that supervised techniques could ...