They are generated when there is a need to answer a specific business question. These reports are useful for obtaining more in-depth information about a specific query. An ad hoc report could focus on your corporate social media profile, examining the types of people who’ve liked your page ...
Biological replicates are indispensable in ATAC-seq protocols to ensure reproducibility and reliability. As with RNA-seq, maintaining high-quality standards, particularly in sequencing depth and raw reads processing, is essential. ATAC-seq vs. RNA-seq: A Contrast While both techniques are predicated o...
No One Is Going to Tell You Feb 25, 2021 BioPharma Reporter German program using whole genome sequencing to diagnose rare diseases and cancer risk Feb 17, 2021 Cambridge Independent Illumina Accelerator Cambridge announces four chosen genomics start-ups and £20m match-funding pledge Feb 17, ...
High throughput sequencing is poised to change all aspects of the way antibodies and other binders are discovered and engineered. Millions of available sequence reads provide an unprecedented sampling depth able to guide the design and construction of effective, high quality naïve libraries containing...
Prior to DNA methylation sequencing, researchers treat their samples with sodium bisulfite to distinguish methylated cytosine from unmethylated cytosine.
NGS detection is directly targeted to one hundred known genes for genome sequencing, to test microsatellite instability in tumor tissues. In 2017, MSK’s IMPACT products were approved to detect microsatellite instability in cancer tissues. In comparison with traditional methods, the uniformity of check...
Provides a quick introduction of VAF (Variant Allele Frequency), which is defined as the ratio of variant reads over read depth using NGS (Next-Generation Sequencing) terminologies.
sequencing, the shotgun metagenomic approach is applied to DNA from samples, however, the sequencing depth targets of these samples are much lower than standard metagenomic sequencing. In doing so, the amount of data generated for each sample is reduced while also reducing the sequencing costs per...
A greater depth of coverage was obtained by multiplexing six negative samples (instead of 48) from segmental NF1 patients in one NGS run. This approach allowed the identification of one mosaic NF1 point mutation with a B7% minor allele frequency. As a more important sequencing depth is needed...
Genome sequencing, gene annotation, and the activation of silent gene clusters constitute the basis of new methods for massive screening and new antibiotics discovery and yet success is limited [9]. Aeromonas strains are known to produce several antimicrobial substances with the potential to become ...