Assuming the genome is a completely random assortment of nucleotides, how many DNA bases would one or more transcription factors have to bind in order to select a unique site in the genome? How much DNA is there in the human body? The nuclear genome of yeast is ...
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followed by alignment of reads to a reference genome or assembly of reads for novel genomes, and finally by variant calling. The main output is a BAM file containing aligned reads. Tertiary analysis is the most challenging step, as it...
We expand the genome size (GS) database for Bromeliaceae, specifically for subfamily Pitcairnioideae, and verify whether GS can provide information on the diversification of the five genera in this subfamily. We also provide a phylogenetic perspective on GS evolution in ...
genome, or gene expression assays to the transcriptome. A web-based tool that can be used in verification of coordinates is the UCSC Genome Browser In Silico PCR utility at: genome.ucsc.edu/cgi-bin/hgPcr. It is also important to make sure that amplicons are al...
they are typically defined as SNVs that occur in >1% of the population. Based on the number of SNPs cataloged in the SNP database (dbSNP) [1] and a haploid genome size of 3.1 x 109 bp [2], the human genome should contain a SNP approximately once every 9.5 bases. Other common mode...
Dark DNA is the genetic code in the human genome that currently has no known original source. We have traced 8% of the human genome to viruses. 1-2% of our genome comes from other animals. Believe it or not you share 55% of your genetic code with a banana – and that’s not just...
Gene and genome duplications provide a source of genetic material for mutation, drift, and selection to act upon, making new evolutionary opportunities possible. As a result, many have argued that genome duplication is a dominant factor in the evolution of complexity and diversity. However, a clea...
“snips”) are single base-pair changes in DNA that occur at specific places in the genome. For example, most individuals carry the C nucleotide at a specific base position in the genome, but in a minority of individuals this is replaced by an A...