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When you firstlearn flow cytometry, you were likely told the misleading phrase, “Forward scatter signal intensity is proportional to cell size, and side scatter signal intensity is proportional to cell granularity.” The short story here is that, yes, thiscanbe the case when we’re de...
What do Flow Cytometry Users Have to Say?Erica Tennenhouse
By forcing cells one at a time through a similarly sized aperture between two electrodes passing a charge, when the cell comes through, it interrupts the signal, and the cell is counted. The amount of electric resistance also helps calculate the cell’s volume. Flow Cytometry Flow Cytometry ...
Oxidative respiration is the primary mechanism that cells use to release chemical energy stored in nutrients (primarily glucose) to fuel cellular activity. Occurs in mitochondria, and, as its name implies, requires oxygen. Acetyl-CoA is produced from pyruvate molecules generated via glycolysis and ent...
Flow cytometry–based assessment of minimal residual disease (MRD) Minimal residual disease (MRD) is a state where patients still carry small counts of leukaemic cells during and after therapy. These cells can be the source of future recurrence, and monitoring MRD is essential for therapy adjustmen...
Resolved by:Perform and/or document a PNH Flow Cytometry lab test using the Add a lab link within the alert. Headache Quality of Life (QOL) Patient has clinical markers associated with primary headache disorder or migraine and should be assessed at least twice a year. Perform the MIDAS (Migr...
Recent years have seen so-called natural killer T (NKT) cells emerge as important regulators of the immune response. The existence of NKT-cell subsets, and other types of T cell that resemble NKT cells, is an ongoing source of confusion in the literature
and maturation. Specific antibodies to these markers can be used to identify individual B cell types by immunohistochemistry (for example, in the tumor microenvironment) or to quantify and isolate them from a heterogeneous pool of immune cells through flow cytometry—a process known as ...
What is “Event Rate”? In flow cytometry, an “event” is defined as a single particle detected by the instrument. Accurate detection of events using flow cytometry requires the ability to separate single cells with specific characteristics from within a heterogeneous ...