The Biotage® PhyPrep fromBiotageis an automated platform for plasmid DNA purification. The system releases laboratory staff from repetitive, manual labour and takes full advantage of the PhyTip® columns’ dual flow chromatography technology, to yield supercoiled, endotoxin-free, tr...
DNA extraction is a critical first step in the experimental workflow of DNA fragment analysis. The overall efficiency, quality, and size of the PCR product can be significantly affected by characteristics of the sample itself and the method chosen for nucleic ...
Product Name: Hipure Circulating DNA Kit; Applications: Qpcr, Liquid or Solid Chip Analysis, Hybridization; Purification Method: Mini Spin Column; Purification Technology: Silica Technology; Process Method: Manual (Vacuum); Sample Type: Serum, Plasma and Other Cell-Free Fluid Samp...
Here is the main steps of NGS process: 1. Library Preparation— A DNA library is prepared from a patient's sample cells, which are randomly broken into a large amount (in millions) of DNA fragments. Amplification, purification, and other treatments are performed increases the efficiency of ...
Sample Prep - Subsequently, DNA sample preparation follows, encompassing tasks like DNA extraction, PCR gene amplification, and DNA fragment purification. Enzyme Digestion - Afterward, the DNA fragments undergo digestion by restriction enzymes, a process that can be automated with robotic pipetting system...
Main Functions:Isolation up to 10mg Endotoxin-Free Plasmid DNA; Applications1:Cell Transfection, Animal Injection, etc.; Purification Method1:Mega Spin Column; Purification Technology:Silica Technology; Process Method:Manual (Centrifugation or Vacuum); ...
The three steps of a DNA ligation reaction (Figure 1): The DNA ligase is self-adenylated by reacting with ATP or NAD+, creating a phosphamide bond between a lysine residue within the enzyme and an AMP molecule of the cofactor, either ATP or NAD+. The adenyl group is transferred to 5...
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Ida Nadi is situated towards the left side and Pingala Nadi towards the right side of Sushumna Nadi. Ida and Pingala nadis spiral around the Sushumna Nadi resembling the double helix of human DNA. They cross each other at certain points along the spine, formingChakras. ...
Prepared RNA samples may need DNase treatment to prevent potential amplification of any contaminating genomic DNA, which could lead to overestimation of the copy number of an mRNA. When starting material is limited, however, DNase treatment may be inadvisable, because the additional manipulation could...