The purified DNA was spotted on Hybond N+ membrane using a slot-blot apparatus and vacuum suction. The membrane was then crosslinked and blocked in 5% milk-TBST, and detected by S9.6 antibody (DNA:RNA hybrid-specific antibody). Chloroplast chromatin immunoprecipitation (cpChIP) cpChIP was ...
The purified DNA sample (T3) from the non-autoclaved group showed a positive band on agarose gel, while the autoclaved sample (T2) was found negative (Fig. S5B). Preparation of water samples Mock samples for screening of optimum incubation temperature were prepared by filtration of 200 ml of...
Optional: Centrifuge the DNA in TE Buffer (TE) for 1 minute at room temperature at high speed to remove any insoluble particles that may be present. Transfer the supernatant containing the DNA into a fresh tube. Store the purified DNA a...
Thermo Scientific Phusion 高保真 DNA 聚合酶为高效 PCR 设立了“金标准”。Phusion高保真了解更多信息 Have Questions? 更改视图 货号颜色反应次数 F530LColorless500 次反应 F530SColorless100 次反应 F-530XLColorless2,000 次反应 3 选项 货号F530L
been purified from different organisms, it is not yet certain whether those that have been most extensively studied are necessarily involved in the formation of new DNA molecules, or whether they are primarily concerned with the repair of damaged regions of molecules. A polynucleotide ligase that ...
Visible PCR bands were purified and cloned into pUC19 vector and sequenced by Sanger sequencing. The sequences were analyzed to identify circularization junction of eccDNAs purified from 1C3-1cells. Results Induction of primary mouse liver cancer by multiplexed CRISPR/Cas9 targeting 34 TSGs To ...
Furthermore, we compared the purification of PCR products obtained from the TPI gene using the freeze-squeeze modified method with five other commercial kits that indicated in their instruction manuals that fragments measuring 50 bp in length can be purified. However, the instructions also mentioned ...
. Next, 1 μg of purified RNA was converted into cDNA using the iScript cDNA synthesis kit (Bio-Rad, #1725037), following the manufacturer’s instructions. qPCR. The mRNA levels of genes of interest were measured by the SYBR green-based real-time qPCR method. For each sample, different ...
A standard PCR was employed to amplify this material, and the gel-purified product was digested with Bbs I and gel-purified again to produce material for another round of selection. A notable difference in this protocol from previous ones [3,7] was the use of heparin after the 1st round ...
and purified by Ultra-Polyacrylamide Gel Electrophoresis. They are dissolved in TE buffer (Beijing Solarbio Science & Technology Co., Ltd.) and stored at −20 °C. The SYBR GreenI (SGI), purchased from MCE (MedChemExpress, USA), is stored at −20 °C. Cytomics FC 500 (Beckman...