DNA is the genetic information carrier molecule in the cell and thus it is very essential to keep the genetic information intact. Even though DNA holds a prime position, it is one of the highly susceptible molecules in the cells because DNA can be damaged by a number of factors both interna...
• If DNA fragments are much larger in size so firstly the gel should be treated with HCl, causes depurination of DNA fragments. • After separating these fragments, placed a nitrocellulose sheet over the separating gel. Apply pressure over the membrane so that proper...
It is important that the DNA sample contains 5–10 mM Tris-HCl buffer with a pH of 8.0–8.5 to prevent depurination. For conventional MLPA experiments, we recommend adding 1 µl of 50 mM Tris-HCl pH 8.5 to 4 µl of sample DNA if it is unknown if sufficient buffering capacity i...
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There are many physical and chemical causes of DNA damage including gamma or UV irradiation, alkylation of bases, DNA crosslinking, depurination of DNA, alteration of the deoxyribose sugar moiety, reaction with oxidative free radicals and more. Each of these types of DNA damage is different and ...