Genotyping is an umbrella term for several methods used to analyze variations (e.g., SNPs, insertions or deletions) in genomes between individual organisms.
Fragment analysis is a powerful research tool that provides relative quantitation, sizing, and genotyping information and enables a wide array of genetic analysis applications. To find out more about the applications enabled by CE, see the articleWhat applications does capillary electrophoresis ...
It creates a translatome, which is a "global snapshot" of all the ribosomes actively translating in a cell at a given time. Researchers can now evaluate the location of the translation start sites, the complement of translated ORFs in a cell or tissue, the allocation of ribosomes on mess...
Currently,Agrobacterium-mediated transformation is the most effective approach for genetically modifying fruit trees and is widely utilized for citrus genetic improvement and functional genome research (Fig.2a–h). Vacuum infiltration, a technique shown to enhanceAgrobacteriuminfection, has been successfully ...
Recent studies of these so-called wild-type GISTs have uncovered a number of other oncogenic drivers, including mutations in neurofibromatosis type I, RAS genes, BRAF, and subunits of the succinate dehydrogenase complex. Routine genotyping is strongly recommended for optimal management of GISTs, as ...
When many different cells in the same organ have different somatic mutations, it is called a mosaic. This can be differences in allele frequency or copy number. Using SNP gene array chips, mosaics can be detected if over 5% of the cells have them. PCR (polymerase chain reaction) or mass ...
ERPs were recorded using 128-channel dense array EEG during an 840-trial arrow-flanker task. Amplitudes and latencies were scored in re- sponse-locked error trials for the ERN and the PE. Results (study two): The data in the normal control subjects showed a relatively late development of ...
Determinants of statistical power such as sample size, disease heterogeneity, pedigree and genotyping errors, as well as the effect of the type and density of genetic markers, are a key factor in genetic studies. Studies should either have sufficient power to detect a small effect size of multip...
We estimated the effect of sexual behavior, age, and immunodeficiency on the number of high-risk human papillomavirus (HR-HPV) types in the anal canal among human immunodeficiency viruspositive men who have sex with men (MSM). Anal samples were genotyped with the Linear Array HPV Genotyping ...
DNA extraction, sequencing and SNP genotyping was conducted by Diversity Array Technologies (DArT, Canberra, Australia). Samples were processed using the proprietary DArT Pty Ltd analytical pipeline, which includes technical replicates from a subset of samples to assess genotyping reproducibility (Georges ...