pBR322是一个人工构建的重要质粒，有万能质粒之称。它是由pSF2124、pMB8及pSC101三个亲本质粒经复杂的重组过程构建而成的。优点 1质粒载体pBR322的大小为4361bp，相对分子质量较小 2它带有一个复制起始位点，保证了该质粒只在大肠杆菌的细胞中行使复制的功能。3具有两种抗生素抗性基因——氨苄青霉素抗性...
What is the function of DNA ligase in recombinant technology? What happens after genetically modified plasmids are inserted into bacteria? Why is this event so rare, that is, why do bacteria take up plasmids so rarely? What antibiotic resistant genes are in pBR322?
When is a High-Copy Number Good? There are several situations where you want a high copy number plasmid, including: Protein expressionAlthough there is no significant advantage to using higher-copy-number plasmids over pBR322-based vectors in terms of protein production yields, a high-copy plasmi...
What is the structure and function of DNA? What does a drawling of a operon turned off look like? What is the name given to a single piece of coiled DNA? What is a nucleotide? The plasmid pBR322 is a closed circular dsDNA containing 4363 base pairs. What is the length in nm of ...
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The key difference between pBR322 and pUC19 is that pBR322 is a plasmid vector that is 4361 base pairs in length, while pUC19 is a plasmid vector that is 2