As circular but not linear pBR322 DNA was actively transcribed by polymerase II in oocytes, even in the presence of SV40 DNA, it is likely that pBR322 competes for transcription factors required for viral gene expression. Injection of pBR322 as early as two hours after injection of SV40 DNA...
In pBR322 plasmid, p represents the name of the researcher who constructed the plasmid.A.正确B.错误的答案是什么.用刷刷题APP,拍照搜索答疑.刷刷题(shuashuati.com)是专业的大学职业搜题找答案,刷题练习的工具.一键将文档转化为在线题库手机刷题,以提高学习效率,是学
Efficient transformation of pBR322 and its derived plasmids, which have been widely used as cloning vectors in Escherichia coli , was observed in Pseudomonas avenae (K1), the pathogen of leaf blight disease in cereals. Moreover, there was a 10- to 50-fold transformation efficiency (1.3–3.0×...
α‐Santonin, a compound extracted from the flower heads of Arthemisia maritima plant, was effective in elimination of small, multicopy, relaxed plasmids (pBR322 and pBR329 with CoLE 1 origin of replication in Escherichia coli , whereas plasmids of IncF 1 , H 1 and X group were totally ref...
Short sequence tracts composed of alternating guanosine and thymidine nucleotide residues poly[d(GT)-d(CA)] carried in a derivative of pBR322 were recombinogenic in a recA host. Recombination brought about by poly[d(GT)-d(CA)] tracts displayed two interesting properties: (i) the reaction was...
A new horizon for pBR322: in vivo insertion of plasmid fragments into wide host range shuttle vectors. Nucleic Acids Res. 23 : 5085-5086.Kok, M. 1995. A new horizon for pBR322: in vivo insertion of plasmid fragments into wide host range shuttle vectors. Nucleic Acids Res. 23:5085- ...
Plasmid pBR322 and its numerous derivatives are used extensively for research and in biotechnology. The tetracycline-resistance (TcR) genes in these plasmids are expressed constitutively and cells carrying these plasmids are resistant to tetracycline. We have shown that expression of the TcR gene has ...
Regulation of replication of plasmid pBR322 in amino acid-starved Escherichia coli strains. Mol. Gen. Genet. 243:374-378.Herman, A, Węgrzyn, A, Węgrzyn, G (1994) Regulation of replication of plasmid pBR322 in amino acid-starved Escherichia coli strains. Mol Gen Genet 243: pp. 374...
Plasmid stability and the activity of (-lactamase were examined in the factorial fed-batch cultures of E.coli HB101(pBR322) in a fermentor. No loss of plasmid pBR322 was observed when temperature shifted from 33 oC to 39 oC, pH from 6.4 to 7.2, dissolved oxygen (DO) from 40% to 80...
M: pBR322-HaeIII digest molecular weight marker (Boehringer). analysis. The two Greek populations were charac- terized by a lower level of genetic variability, mainly because of the absence of rare alleles in these popula- tions. However, differences in the distributions of rare alleles were ...