Define "primer self-complementarity" and explain what is the phenomena of primer loop and primer dimer in PCR. Why do you send the sample to the centrifuge twice during the purification PCR procedure? How does vertical gel electrophoresis differ from horizontal gel electrophoresis? What i...
When your blood goes through the centrifuge, a spinner, the blood cells spin to the bottom to make a sediment, and the clear liquid on top is called plasma. The laboratory technicians will put the sediment under a microscope and will examine it and count how many red blood cells, white ...
What is the purpose of boiling your protein samples before loading them into the polyacrylamide gel? Polyacrylamide Gel: Polyacrylamide gel electrophoresis is a method for separating biological macromolecules based on their electrophoretic mobility and is used in bioch...
The scientific study of matter's characteristics and behaviour is known as chemistry.andare different from the normal vocabulary. It is a branch of natural science that examines the building blocks of matter, including the atoms, molecules, and ions that make up compounds and their composition, s...
1. What purpose does the loading dye serve? 2. What is the purpose of the molecular weight ruler? 3. Analyze whether the DNA extraction column used was a cationic or anionic exchange chromatography method and state the reason why. What is the concept of negative staining? How does it...
Why do responsible scientists avoid using the term "scientific proof"? Explain. Why don't spiders run out of silk? Explain why an elevation of a serum enzyme may indicate a disease state. What is the main purpose of EDTA in DNA extraction? What was the purpose of Mendel'...
What is the effect of primer length on the specificity of resultant PCR products? What should the Ct values of a negative control be from an RT-PCR? What is the main purpose of EDTA in DNA extraction? What are the probable reasons for getting a smudged effect when you run agarose ...
In that case, they should be frozen or possibly fixed in formalin, as it has been shown that diatoms can be identified even in the latter case [30,31,32]. 3.1.2. Centrifugation and Filtration To separate diatoms from the water samples, it is considered suitable to centrifuge the sample ...