ExtractionProteinase KTEMPERATURESAMPLESTOOLIn the biomonitoring using environmental DNA (eDNA) analysis, the suppression of eDNA degradation has been an important issue to estimate accurately the presence of target species and their eDNA concentrations. A recently proposed eDNA preservation technique requires...
min (during DNA extraction). The results of these sequentially executed processes were analyzed using gel electrophoresis. Thus, this work demonstrates the adaptability of the system to replace many common laboratory tasks on a single platform (through re-programmability), in rapid succession (using ...
Multiple DNA Extractions Coupled with Stable-Isotope Probing of Anthracene-Degrading Bacteria in Contaminated Soil In many of the DNA-based stable-isotope probing (SIP) studies published to date in which soil communities were investigated, a single DNA extraction was pe... Maiysha,D.,Jones,... ...
A simple and rapid microscale technique is described for the isolation of plasmid DNA which involves cell lysis with phenol, centrifugation, phenol extraction, ethanol precipitation, and RNase digestion. The plasmid DNA is of suitable pu... RD Klein,E Selsing,RD Wells - 《Plasmid》 被引量: 28...
Centrifugationcan be applied to provide the necessary pressure difference in filtration (see rel.7.2.3) or for the separation of the layers in liquid-liquid extraction. For example, a device that can be applied in solvent extraction is the centrifugal separator [22], [23]. ...
Glencoe Biology: Online Textbook Help Browse by Lessons Extraction in Chemistry | Process, Types & Example Create an account to start this course today Used by over 30 million students worldwide Create an account Explore our library of over 88,000 lessons Search Browse Browse by subject Pl...
pellet compared to the fat fraction (P < 0.04), and three low-abundance species (< 5% relative abundance) were recovered from one fraction only. However, inclusion of fat reduced the ef f i ciency of DNA extraction by 39%. Culture-based methods were used to quantify the distributi...
salt of a heavy metal (when a DNA component migrating in a centrifugal field reaches a point in such a gradient where the DNA and gradient densities match, the DNA stops moving, i.e., it attains equilibrium, and its position in the gradient is largely determined by its base composition)....
Proteoglycans were isolated from cartilage by extraction with 4M-guanidinium chloride followed by direct centrifugation in 4M-guanidinium chloride/CsCl at a low starting density, 1.34 g/ml. N-Ethylmaleimide was included in the extraction... D Heineg?Rd,M Paulsson,S Inerot,... - 《Biochemical Jo...
include, but are not limited to, high temperature extraction, filtrationandcentrifugation,distillation, and crystallization. legco.gov.hk legco.gov.hk 可用作提煉 食物或配料的程 序 , 包括( 但 不局限 於 ) 高溫抽取 、過濾及離心作用、蒸餾,以及晶 化。