Primers should be specific and should not be able to bind elsewhere in the template DNA, to the probe, or to each other. The Tm of TaqMan probes should be ~10°C higher than the Tm of the primers (approximately 68–70°C). If you are currently using TaqMan predesigned assays containi...
What does enzyme binding affinity mean? The use of enzymes can be widely seen in the beer brewing process, cosmetic production line, and pharmaceutical production lines. They are proteins, which upon interacting with the interspecific substrates, function as superior catalysts. This is also true of...
Primers should be specific and should not be able to bind elsewhere in the template DNA, to the probe, or to each other. The Tm of TaqMan probes should be ~10°C higher than the Tm of the primers (approximately 68–70°C). If you are currently using Ta...
DNA replication is defined as a process by which a DNA molecule is copied and replicated into two, same DNA molecules. DNA replication is thus, the process of DNA copying and duplication. The process of DNA replication will take place in a semi-conservative manner....
What does mRNA bind with in the cytoplasm?Messenger RNA:Messenger RNA (mRNA) is a type of RNA that is a copy of a specific gene in DNA. mRNA carries the instructions for protein synthesis to other organelles in the cell.Answer and Explanation: ...
E.coli DNA ligase uses NAD+ as a cofactor to bind cohesive double-stranded DNA fragments’ ends. E.coli DNA ligases need unique chemical conditions in order to ligate blunt end DNA fragments, and generally it does not target DNA-RNA or RNA-RNA duplexes efficiently [2]. The T4 DNA ligase...
What does DNA ligase do during DNA replication, and what is DNA ligase? Which one is mismatched? a. DNA polymerase III - adds nucleotides to an RNA primer b. Ligase - seals any nicks in the new sugar/phosphate backbone c...
‘religion', you mean does it have priests and churches and a band of followers who are prepared to kill non-believers, then the answer is no. if, on the other hand, you refer to the original, literal meaning of the word, namely to ‘bind again', to reunite the mistaken person that...
Once the APC comes in contact with a T cell expressing the corresponding TCR, the antigen-MHC complex will bind to the TCR and initiate the signaling cascade. This alone is not sufficient to activate most naïve cells and can cause a cell to become anergic, or unable to carry out an im...
In any biological system with memory, the state of the system depends on its history. Epigenetic memory maintains gene expression states through cell generations without a change in DNA sequence and in the absence of initiating signals. It is immensely powerful in biological systems — it adds lon...