What is the function of the Taq DNA poltmearse in a PCR reaction? Describe the steps of Reverse transcription polymerase chain reaction (RT-PCR). What is the difference between degenerate PCR and nested PCR? If
What enzyme is used to link RNA nucleotides to each other during transcription? What are the enzymes involved in DNA replication in prokaryotes? Give the function of each enzyme. Which enzymes catalyze the chain elongation steps during replication, transcription and translation, respectively?
Potential drug–drug interactions mediated by the ATP-binding cassette (ABC) transporter and solute carrier (SLC) transporter families are of clinical and regulatory concern. However, the endogenous functions of these drug transporters are not well understood. Discussed here is evidence for the roles ...
Now that you’re more familiar with the basics of CRISPR and its applications, you’re probably curious about how to use it in your own research. In this section, we’ll take you through the main steps of designing your own experiment. A moredetailed chapter-by-chapter guide for designing...
Take a quick interactive quiz on the concepts in Enhancer in Biology | Definition, Sequence & Transcription or print the worksheet to practice offline. These practice questions will help you master the material and retain the information.
This is because they can go against one of the central dogmas of biology by doing reverse transcription that allows them to transcript from RNA back to DNA, which is the opposite of what other viruses do to generate viral materials within the host. Retrovirus infections are very hard to ...
Telomere biology disorder (TBD). One of a set of pathologies that are defined by the presence of short telomeres. Chromothripsis A mutational phenomenon that involves catastrophic shattering and rebuilding of chromosomes, leading to multiple clustered chromosomal rearrangements. Kataegis Clustered point muta...
ATP is produced through the course of three linked steps. First, using enzymes present in the matrix, pyruvate and fatty acids are converted into a molecule known as acetyl-CoA. This then becomes the starting material for a second chemical reaction known as the citric acid cycle or Krebs Cycl...
Transcription takes place in two broad steps. The first stage involves the pre-messenger RNA being formed with the involvement of RNA polymerase enzymes. Do you remember the rest or do you need a refresher course? Take up this quiz and see just how much you got to understand from class. ...
What are the steps for a qRT-PCR? It is a quantitative reaction time polymerase chain reaction.qRT-PCR: Reverse Transcription, Quantitative Polymerase Chain ReactionqRT-PCR allows for the amplification and quantification of a specified region from a RNA template. In contr...