Cells or tissues are crosslinked to preserve chromatin–protein interactions, and the protein of interest is immunoprecipitated with an antibody. Quantification of the precipitated DNA by PCR or profiling methods indicates enrichments of the protein of interest. Chromatin remodelers Enzymes or complexes ...
Proteomic datasets from HEK293 and braintumor cells are accessible via the surface protein atlas (https://wlab.ethz.ch/cspa/). Proteomic datasets of JK-6L cells are currently unpublished proprietary data available upon request. The relative gene expression data are included in the supplementary ...
Patch-clamp experiments are used to gather data which can answer a large variety of physiological questions, not only in the field of neuroscience. During the last three decades, patch-clamp recordings have also become more important for the investigation of ion channels in non-excitable cells. ...
Our STING Antibodies are available in unconjugated and multiple conjugated formats. STING Antibody [NBP2-24683] Immunohistochemistry analysis of STING protein in formalin-fixed paraffin-embedded mouse lung tissue section using STING antibody at 1:150 dilution with HRP-conjugated secondary. The signal was...
Acta Pharmacologica Sinica npg 94 www.nature.com/aps D'Onofrio PM et al While necrosis is more dominant in the ischemic core, apop- tosis becomes more common in the penumbra as the cells are found further away from the core[2]. Apoptosis is a normal process during development, and is ...
Binding studies in Xenopus oocytes and transfected HEK-293 cells revealed that a CFTR peptide fragment that binds AMPK (CFTR-1420-57) disrupted the AMPK-CFTR interaction. Introduction of CFTR-1420-57 into human bronchial Calu-3 cells enhanced forskolin-stimulated whole cell conductance in patch ...
In the top 100 list there are five cell lines derived from HEK293 cells, together totalling a >10% share of the citations in 2021. Other cell lines featuring in the top 100 include VERO lineage cells and HeLa cells. VERO cells took second and third place in the top 100 list in 2021...
Human embryonic kidney 293 cells frequently used in cell biology and biotechnology for transfection and production of viral vectors, vaccines, and recombinant proteins. Bacterial mechanosensitive channel of large conductance. Bacterial mechanosensitive channel of small conductance. Neuro2a cells are a fast-...
Class I is characterized by four ubiquitous and relatively small enzymes (∼500 amino acids) essentially located in the nucleus of cells [25, 26]. HDACs 1 to 3 are found in complexes with specific transcriptional co-repressors, blocking the expression of tumor suppressor genes [27]. Interest...
Thus, 90% of the glomerular filtrates are normally reabsorbed along the nephron, but this is the net result of reabsorption and secretion in the proximal tubule. Figure 2 illustrates major urate transporters in the proxi- mal tubule. Considering the usual ranges of frac...