(a) What is the name of the DNA duplication process? (b) What is the main enzyme that is involved in it? What are the actual steps in DNA replication? What enzymes are required and what are their functions? What are DNA ligases? How do these enzymes participate in recombin...
What are the advantages and disadvantages of this technology? What is DNA technology and how is it used? What are the different uses of recombinant DNA? What makes biotechnology different from recombinant DNA technology? What are DNA ligases? How do these enzymes participate in recombinant DNA tec...
Unlike the E.coli DNA ligase, the T4 DNA ligase uses ATP as a cofactor. As the name implies, thermostable DNA ligases function at higher temperatures than the two previously discussed ligases. This is because they are enzymes that are found in microbes that thrive in extreme temperatures like...
Ligases are crucial for connecting Okazaki fragments on the lagging strand during DNA replication, ensuring the continuity and integrity of the newly synthesized DNA strand. 14 Can ligases work without ATP? No, ligases require ATP or a similar energy source to catalyze the bonding of molecules, as...
Ligases:Ligases catalyze the joining together of two separate molecules into one molecular compound, releasing energy during the process. Example: DNA ligase, which forms a phosphodiester bond that joins two fragments of DNA. Enzymes Amplite® Fluorimetric Alanin...
How are polymers classified according to their structure? Give important features of each class. Also give two examples of each class. View Solution Explain briefly : (a) Restriction enzymes (b) Recombination DNA View Solution Discuss the classification of hydrocarbons. Give two examples of each ty...
View Solution Molecular scissor is: View Solution What are Molecular Scissors? Give one example. View Solution Exams IIT JEE NEET UP Board Bihar Board CBSE Free Textbook Solutions KC Sinha Solutions for Maths Cengage Solutions for Maths DC Pandey Solutions for Physics ...
serves as a coenzyme in redox reactions. It acts as a donor of ADP-ribose moieties in ADP-ribosylation reactions and a precursor of cyclic ADP-ribose, a second messenger molecule. β-Nicotinamide Adenine Dinucleotide is also a substrate for bacterial DNA ligases and a group of enzymes called ...
What are the two types of enzymes? Enzymes are classified into six categories according to the type of reaction catalyzed:Oxidoreductases, transferases, hydrolases, lyases, ligases, and isomerases. Structurally, the vast majority of enzymes are proteins. Also RNA molecules have catalytic activity (...
What are DNA ligases? How do these enzymes participate in recombinant DNA technology? In DNA of E Coli has 20% bases of guanine, what will be a fraction of thymine? a) 30 b) 3 c) 15 d) 5 In PCR, what would be the most likely outcome if annealing temperatures of a) 45C or b)...