A negative result means there are no bands on the blot. An indeterminate result is anything in between, commonly an isolated p24 band. If a WB is reported as indeterminate, then additional testing such as a p24 antigen ELISA, or an approved HIV-1 RNA assay like the Gen-probe® APTIMA...
Western blot analysis.Western blot was performed as previously described. Rats were anesthetized with isoflurane at 24h after SAH and transcardially perused with 100 ml of chilled PBS (0.01 M, pH7.4) before brains collection to ensure that there were no blood contaminants. The left hemispheres we...
PCR and Western blot were used to select the transgenic tobacco plants. ELISA was used to evaluate the expression level while colony forming assay was used to test its biological activity. 蛋白印迹实验鉴定转基因植株,ELISA方法测定重组蛋白表达水平,集落形成实验测定重组蛋白活性。相关...
Veterinarians diagnose EPM by performing a neurological examination and by ordering Western blot tests for antibodies to S. neurona in the blood and/or cerebrospinal fluid (CSF). The negative predictive value of the Western blot test is generally accepted to be high for both serum and CSF. If...
that HIV-2 Western blot be included in the diagnostic algorithm for HIV-1/2 to followup negative or indeterminate HIV-1 Western blot results.doi... DF Díaz,E Ortiz,D Martín,... - 《Medicc Review》 被引量: 5发表: 2012年 Anti–HIV indeterminate Western blot in dialysis patients: A lo...
Further known positive and negative controls make up 5-10% of the blots on each gel and they must be accurate before results are released. Trained US Army professionals reexamine and certify all blots. In addition a professional must draw a 2nd sample and that sample must be confirmed to be...
The meaning of WESTERN BLOT is a blot consisting of a sheet of nitrocellulose or nylon that contains spots of protein for identification by a suitable molecular probe and is used especially for the detection of antibodies.
n.Western Blot, “inmunoblo'', prueba subsecuente para confirmar la infección por el virus VIH, en pacientes con evidencia de exposición, indicada por un ensayo enzimático inmuno-sorbente (ELISA). English-Spanish Medical Dictionary © Farlex 2012 ...
I used the iBlot™ 2 Dry Blotting System and noticed that the signal intensity was similar for different protein loads after detection. Why is this? This is most likely due to the protein load being too high, as...
Results In 3 494 cases of WB test, there were 1 822 samples confirmed to be HIV-1(+), accounting for 52.15%, 416 samples were uncertain, accounting for 11.91%,1 256 samples were negative, accounting for 39.95%. In HIV-1 antibody positive samples, the frequency of gp120, gp160, p24 ...