Western blot analysis of EGFR was performed by loading serially diluted A431 cell lysate from 20 µg to 312 ng per well onto a Novex™ 4–20% Tris-Glycine gel, WedgeWell™ format (Cat. No. XP04202BOX). Proteins were transferred using Invitrogen™ iBlot™ 2 Transfer Stacks, nitro...
首先，打开Image J软件，启动界面如图1所示。选择“File”>“Open”，找到你需要分析的Western blot图片，如内参β-ACTIN，如图2所示。将图片转换为8位灰度图，操作路径为“Image”>“Type”，如图3所示。接下来，对背景进行统一处理。点击“Process”>“Subtract Background”，选择rolling ball radius为5...
The negative predictive value of the Western blot test is generally accepted to be high for both serum and CSF. If the agreement between serum and CSF test results is strong, serum tests could be used to substitute for CSF tests in some cases. The purpose of this study was to assess the...
Far–western blot analysis is an alternative method to analyze protein–protein interactions that entails probing proteins that have been separated by gel electrophoresis with tagged bait proteins, and then detecting the interacting proteins (...
3.目的蛋白含量是否达到wb的检测限?(目前认为Western Blot的有效检测下限为0.1-1ng,含量高有助于条带显影) 如何确认目的蛋白的抗体? 1.明确目的蛋白名称或者目的基因名称、明确样品种属来源; 2.在各个抗体或试剂公司网站进行搜索; 3.选择抗体的反应性(样品是什么种属来源就选择什么物种); ...
Objective To understand the distribution of western blot(WB) bands of male drug addicts infected with HIV-1 virus in Huizhou City.Methods All the HIV-1 antibody positive samples of drug addicts from 2007 to 2010 in our laboratory were collected and the WB bands were analyzed.Results Among the...
中文标准名称:western Blot试验检测疯牛病病原的方法 标准类型:国标计划 发布日期:1999/12/31 12:00:00 实施日期:1999/12/31 12:00:00 归口单位:全国动物卫生标准化技术委员会 主管部门:农业农村部 起草单位:北京检验检疫局 相近标准:SN/T 2206.7-2010 化妆品微生物检测方法 第7部分:蛋白免疫印迹法检测疯牛病...
Objective:To understand the maternal HIV-Ab test indeterminate results,analysed the indeterminate result of 8 cases of maternal human immunodeficiency virus(HIV) antibody confirmatory test(WB).Methods: Colleced blood samples of those 8 cases of uncertainty maternal for ELISA,Western blot test(WB) in...
Box 844, Ames, Iowa 50010 2Received 17 July 2007/Returned for modif i cation 18 September 2007/Accepted 16 October 2007We analyzed the performance of a single-band Western blot (WB) test using recombinant p26 (rp26) capsidprotein of equine infectious anemia virus. According to the results ...
Go beyond Western blot or other protein analysis tools. Multiplex chemiluminescent and fluorescent assays on the same sample at the same time.