labeled with 4sU (200 μM, 2 h) and chemically treated with IAA exhibited high T-to-C substitution rates. Other cells without metabolic labeling or IAA treatment showed negligible substitution rates, indicating a high signal-to-noise ratio for Well-TEMP-seq. After 2 h labeling of ...
The N2 chip reduces reaction volume to <30 nL and increases capacity to >240 single cells on a single microchip. The tandem mass tag (TMT) pooling step is simplified by adding a microliter droplet on the nested nanowells to combine labeled single-cell samples. In the analysis of ~100 ...
The Atribacterota are widely distributed in the subsurface biosphere. Recently, the first Atribacterota isolate was described and the number of Atribacterota genome sequences retrieved from environmental samples has increased significantly; however, thei
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tested in the first-round screening. The results showed that our optimized full-component compound had superior ability to induce P19 cells to differentiate into cardiomyocytes than that of the positive control group. In the second-round screening, seven compounds (labeled as “Compound-bcdefg”, ...
Diagram of proposed mechanism of Nef toxicity for neurons cultured in atmospheric oxygen as well as physiological (2%) oxygen indicating the pathway to repair the oxidatively damaged proteins or neuronal death, respectively, beginning with alterations in the native mitochondrial structure, function and ...
The effects of silica nanoparticles on the shape of erythrocytes were studied using a microscope. Photographs taken during particular stages of the experiment illustrated the changes in red blood cells caused by the presence of the compounds analyzed herein (in particular, changes in the shape of er...
All measurements were performed in an aqueous system at room temperature and a measurement angle of 90° to the incident laser beam. The effective diameter (Dh) was calculated from the average of five measurements on each sample. Transmission electron microscope (TEM) images were obtained using a...
The embedded tissue was trimmed and then installed on a microtome for sectioning. The 5 μm-thick tissue sections were used for hematoxylin-eosin (H.E.) staining following the manufacturer’s instructions. Stained glass slides were examined under an optical microscope (Olympus, DP72, Tokyo, ...
The hydration process and microstructure of cement paste were analyzed by hydration heat measurement system, X-ray diffraction (XRD) and scanning electron microscope (SEM). The experimental results show that the ultrafine calcium silicate has a certain impact on the rheology and thickening time of ...