For the photoconversion, we used a confocal microscope (TCS SP8; Leica) with a glycerol emersion 63 × /1.30 lens. Photoconversion can be achieved by scanning with a 405 nm laser. To photoconvert in the local area, we use the region of interest (ROI). Ca 2+ imaging SAGFF(LF)...
A CiliaQ analysis using data from a spinning-disk confocal microscope has recently been published [34]. Fig. 1 CiliaQ workflow. a CiliaQ constitutes a three-step workflow based on three ImageJ plugins: CiliaQ Preparator, which automatically segments the image into ciliary and background voxels...
An example would include combined labeling of the sample by fluorophore- and gold nanoparticles-labeled probes. Such a sample could be studied with the same light source and a single emission filter. Our device can also convert a transmission light microscope with regular transmission objectives into...
3D time-lapse imaging of fluorescently labeled nuclei has allowed the spatio-temporal positions of cells to be tracked, and this has helped to explain targeting phenomena in terms of cellular dynamics [3]. Thus far, nuclei tracking had been performed manually; however, in recent years, computatio...
Before measurement, 40–60 μL of the protein solution was made up to 100 μL with PBS and applied directly onto a template-stripped gold surface mounted onto a microscope slide. Force-extension profiles were accumulated after thermal equilibration at a constant approach speed of 700 nm s−1...
First, the nanoindentation and atomic force microscope 150 Page 14 of 16 Materials and Structures (2020) 53:150 (a) 0.04 0.035 0.03 32 x 32 x 32 subvolume 1 (b) 0.05 0.8 0.04 40 x 40 x 40 subvolume 1 0.8 0.025 0.02 0.015 0.01 0.005 Matrix (1) Inclusion (2) Inclusion (3) YM ...
In contrast, labeled L-ABD(KC) showed weak binding to the Sepharose 6B-CL beads. Since it took an hour from the time of loading L-ABD(KC) to the column to the time of mounting the beads for fluorescence microscopy (time zero shown in Fig. 4), L-ABD(KC) had infiltrated into the ...
Poly(dimethylsiloxane) (PDMS) is likely the most popular material for microfluidic devices in lab-on-a-chip and other biomedical applications. However, the hydrophobicity of PDMS leads to non-specific adsorption of proteins and other molecules such as th
They simply sandwiched double-sided adhesive Kapton tape and polydimethylsiloxane spacers between glass microscope slides using rapid prototyping to fabricate the microfluidic device. This system was used for the detection of simulated viral phage DNA. A direct comparison between the oscillatory-flow ...
(subtype: mesenchymal) were recuperated after day 21, fixed and labeled for nestin (marker of neural stem cells) and GFAP (marker of glial cells). As seen inFigure 3D cells were labeled with nestin or GFAP and some were labeled with both nestin and GFAP, signifying the presence of a ...