Figure 3.A diagram displaying the optical setup for a MUSE (microscopy with UV surface excitation) microscope. The tissue is supported on a UV-transparent window connected to a XYZ-stage. A 280-nm LED excites the specimen obliquely, generating visible-range emission that is directed by a conve...
Before measurement, 40–60 μL of the protein solution was made up to 100 μL with PBS and applied directly onto a template-stripped gold surface mounted onto a microscope slide. Force-extension profiles were accumulated after thermal equilibration at a constant approach speed of 700 nm s−1...
In contrast, labeled L-ABD(KC) showed weak binding to the Sepharose 6B-CL beads. Since it took an hour from the time of loading L-ABD(KC) to the column to the time of mounting the beads for fluorescence microscopy (time zero shown in Fig. 4), L-ABD(KC) had infiltrated into the ...
Poly(dimethylsiloxane) (PDMS) is likely the most popular material for microfluidic devices in lab-on-a-chip and other biomedical applications. However, the hydrophobicity of PDMS leads to non-specific adsorption of proteins and other molecules such as th
A CiliaQ analysis using data from a spinning-disk confocal microscope has recently been published [34]. Fig. 1 CiliaQ workflow. a CiliaQ constitutes a three-step workflow based on three ImageJ plugins: CiliaQ Preparator, which automatically segments the image into ciliary and background voxels...
This technique is based on the amplification of complementary DNA (cDNA), synthesized from a conserved region of the viral genome, in a PCR reaction using primer pairs and a dual-labeled fluorophore-quencher probe targeting the cDNA sequence of interest. During the amplification cycles the probe ...
microscope. A filter is used which reflects the laser light and passes the fluorescence light. Wolf et al. does not disclose using 5-cyano-2,3-ditolyl tetrazolium chloride (CTC) to detect respiring bacteria. Weaver et al., U.S. Pat. No. 4,959,301 teaches a process for rapidly ...