Figure 3.A diagram displaying the optical setup for a MUSE (microscopy with UV surface excitation) microscope. The tissue is supported on a UV-transparent window connected to a XYZ-stage. A 280-nm LED excites the specimen obliquely, generating visible-range emission that is directed by a conve...
A CiliaQ analysis using data from a spinning-disk confocal microscope has recently been published [34]. Fig. 1 CiliaQ workflow. a CiliaQ constitutes a three-step workflow based on three ImageJ plugins: CiliaQ Preparator, which automatically segments the image into ciliary and background voxels...
In contrast, labeled L-ABD(KC) showed weak binding to the Sepharose 6B-CL beads. Since it took an hour from the time of loading L-ABD(KC) to the column to the time of mounting the beads for fluorescence microscopy (time zero shown in Fig. 4), L-ABD(KC) had infiltrated into the ...
Before measurement, 40–60 μL of the protein solution was made up to 100 μL with PBS and applied directly onto a template-stripped gold surface mounted onto a microscope slide. Force-extension profiles were accumulated after thermal equilibration at a constant approach speed of 700 nm s−1...
3D time-lapse imaging of fluorescently labeled nuclei has allowed the spatio-temporal positions of cells to be tracked, and this has helped to explain targeting phenomena in terms of cellular dynamics [3]. Thus far, nuclei tracking had been performed manually; however, in recent years, computatio...
Poly(dimethylsiloxane) (PDMS) is likely the most popular material for microfluidic devices in lab-on-a-chip and other biomedical applications. However, the hydrophobicity of PDMS leads to non-specific adsorption of proteins and other molecules such as th
(LMP) agarose (Sigma-Aldrich) in H2O. For the photoconversion, we used a confocal microscope (TCS SP8; Leica) with a glycerol emersion 63 × /1.30 lens. Photoconversion can be achieved by scanning with a 405 nm laser. To photoconvert in the local area, we use the region of ...
Whole biospheres prepared with GBM69 PDCs (subtype: mesenchymal) were recuperated after day 21, fixed and labeled for nestin (marker of neural stem cells) and GFAP (marker of glial cells). As seen in Figure 3D cells were labeled with nestin or GFAP and some were labeled with both nestin...
They simply sandwiched double-sided adhesive Kapton tape and polydimethylsiloxane spacers between glass microscope slides using rapid prototyping to fabricate the microfluidic device. This system was used for the detection of simulated viral phage DNA. A direct comparison between the oscillatory-flow ...
Figure 2 provides a transmission electron microscope (TEM) image of the functionalized nanoparticles, vividly illustrating the successful and uniform coating of silica onto the CNTs. The silica grew uniformly along the CNTs, forming a distinct core–shell structure with excellent bonding. Initially, ...