Answer to: Explain the purpose of using a washing buffer in RNA extraction and explain how an elution buffer differs from a washing buffer. By...
Regardless of the wash methods you use, you should use consistent wash conditions: maintain consistent buffer temperatures, wash incubation times, and rotation speeds of any apparatus used for washing. In certain cases, background signals may be r...
In particular, the present invention addresses the laborious and time-consuming aspect of using different wash and elution buffer solutions during multiple, purification steps by using a single wash and elution buffer for nucleic acid purification. The use of a single wash and elution buffer solution...
The partial elution of IgG observed at high pH 11.5 could be attributable to structural changes also suggested for the elution mechanism by acidic buffers40,41,42. As the alkaline buffer did not elute the IgGs, we initially expected recoveries of the IgGs from cultured medium purified by ...
圣尔IP&co-IP裂解、结合、清洗 buffer是一种比较温和的细胞组织裂解液。该产品裂解得到的蛋白样品可以用于常规的PAGE、Western、免疫沉淀(immunol precipitation,IP)、免疫共沉淀(co-IP)和ELISA等。 本产品可以用于动物、植物的细胞或组织样品,也可以用于真菌或细菌样品。
Nucleic acid was isolated from 200 µL of sample on an EZ1 Advanced XL workstation using the EZ1 Virus Mini Kit v2.0 (Qiagen, Hilden, Germany) with 100 µL elution buffer. A defined amount of MS2 bacteriophages was added during extraction and served as an internal control for ...
To test whether the drop in the population of active cells was due to the difference in the organic content between the original and the modified elution buffer, cells extracted from the sediment particles using the modified buffer were incubated with either sucrose or triton X-100 for 10–60 ...
The mobile phase was pumped with a linear gradient program of 3 effluents: chloroform, methanol, and triethylamine buffer. The elution changed from 87.5:12:0.5 (vol:vol:vol) at t = 0 min to 2.0:90:8.0 (vol:vol:vol) after 12 min. This condition was maintained until t = 18 min, and...
Separated proteins were electroblotted (31) onto polyvinylidene difluoride membranes (Immobilon-P, Bedford, MA) with 25% (v/v) ethanol being substituted for methanol in the buffer. The paper was cut in 2-mm strips and incubated with primary chicken antibodies overnight, secondary rabbit anti-chi...
elution buffer liquid discharge device; lightweight material is compressed air bubbles to the top of the upper chamber grille, heavy materials sink into the bottom of the lower chamber, washing water inlet device to ensure the stability of the water level inside the tank washing, elution buffer ...