GST Bind 纯化试剂盒 产品说明书
层析约需要15倍体积GST Bind/Wash Buffer,而批次纯化约需要26倍体 积。1倍体积是指柱床体积(如100µl树脂浆的柱床体积为50µl)。 2. 制备含有100mM还原型谷胱甘肽的10×GST Elution Buffer是将1g还原型谷胱甘肽溶于32.5ml 10× Glutathione Reconstitution Buffer。10×GST Elution Buffer制备好后应该以操作需...
产品名称:GST*BIND BUFFER KIT .0订购此产品 供应商:Merck 规格:*** 目录价:1303 库存状态:3周到货 CAS编号: 应用范围:生化实验 种属来源: 相关信息: GST•Bind™ Buffer Kit The GST•Bind Buffer Kit contains a set of pretested buffers for binding, washing and elution of GST•Tag fusion pr...
ViraBind AAV Purification Kit 产品说明书 Product Manual ViraBind™ AAV Purification Kit Catalog Number VPK-140 10 preps FOR RESEARCH USE ONLY Not for use in diagnostic procedures
While some protocols recommend including a small amount of imidazole in the binding buffer, adding too much can inhibit binding since the imidazole might outcompete the his tag for binding to the metal. The optimal imidazole concentrations used for binding, wash, and elution buffers are very...
4. After incubation, wash the two connected columns with the buffer used to equilibrate the Benzamidine column. The purified, de-tagged protein will flow through both columns. Another advantage to using this procedure is that it avoids imidazole elution and subsequent buffer exchange....
• 2 × 125 ml 4X Ni-NTA Bind Buffer • 125 ml 4X Ni-NTA Wash Buffer • 50 ml 4X Ni-NTA Elute Buffer Store the unopened Ni-NTA Buffer Kit at room temperature. The Ni-NTA Buffer Kit contains 4X concentrated solutions for protein binding, washing and elution under native ...
Omega Bio-tek Mag-Bind
After appropriate washes with selection buffer (see Figure 1B), bound peptides were eluted with elution buffer (4 M urea, 0.5% SDS), desalted, precipitated, and subsequently PCR amplified. Sequence Analysis Amplified DNA from round 10 was digested with restriction enzymes KpnI and BamHI (New ...
The beads were washed three times with 0.9 ml of CLIP lysis buffer. On the last wash, a 10% aliquot was taken from each immunoprecipitation for protein analysis. Beads and half of the input material taken for RNA analysis were subsequently supplemented with 0.2 ml of CLIP elution ...