VSV-G,也就是Vesicular stomatitis virus G protein,它可是分子生物学和生物医学研究中的明星工具哦。它的主要工作就是帮我们把外源基因导入到目标细胞里。VSV-G通过与细胞膜融合,就像一个快递员,把载有目标基因的质粒安全地送到细胞里面。具体来说,VSV-G蛋白会先和细胞膜上的受体结合,然后变个魔术,被细胞透明...
VSV-G (Vesicular stomatitis virus G protein)是一种用于分子生物学和生物医学研究中的实验工具,其主要用途为将外源基因导入目标细胞中。VSV-G膜融合机制是指VSV-G通过与细胞膜融合,将载有目标基因的质粒转运进入细胞的过程。 VSV-G是一种质膜蛋白,包含有融合活性的抗原ic及针对ic的抗体。当将载有目标基因的...
A chimeric protein that is a fusion construct of a series of functional domains is used to deliver a therapeutic agent to a human subject suffering from disease. In some embodiments, the chimeric protein includes a therapeutic region, a transportation region, and a cleavage region disposed between...
其中，立早蛋白（基因）产物包括转录因子（tranion factor），DNA结合蛋白（DNA binding protein）、分泌...
其中，立早蛋白（基因）产物包括转录因子（tranion factor），DNA结合蛋白（DNA binding protein）、分泌...
The high transfect efficiency achieved by concentrated recombinant pseudotyped retrovirusesmay lay a solid foundation for labelingof tissue engineering seed cells.Key words: vesicular stomatitis virus glycoprotein; green fluorescence protein; transfection; pseudotype virus; seed cell 随着组织工程学理论和技术的...
VSV-G is a vesicular stomatitis virus G (VSV-G) protein fragment. VSV-G protein is commonly used in biomedical research to pseudotype retroviral and lentiviral vectors, conveying the ability to transduce a broad range of mammalian cell types with genes of interest. ...
VSV-G,来源于水泡性口炎病毒的融合性外壳G糖蛋白,常被用于逆转录病毒和慢病毒载体的生物医学研究。VSV-G标签通常融合于目的蛋白的N-或者C-端,以便使用免疫组化方法来进行观察和分析。 Fig.1.Immunofluorescence staining (1:1,000) of VSV-G fusion protein in 293 cells with red and counterstained with ...
coprotein(VSV2G),whichcouldbeeffectivelyconcentratedbyultracentrifugation.MethodsPT67packagingcellsweretransfected withplasmidGFP2RVfollowedbyselectionwithG418.Theviral2containingsupernatantsofPT67cellsandtheplasmidcontainingVSV2G genewerecotransfectedintoGP22293packagingcellsthatweretransientlyselectedbyG418.Todeterminevi...
VSV-G,来源于水泡性口炎病毒的融合性外壳G糖蛋白,常被用于逆转录病毒和慢病毒载体的生物医学研究。VSV-G标签通常融合于目的蛋白的N-或者C-端,以便使用免疫组化方法来进行观察和分析。 Fig.1.Immunofluorescence staining (1:1,000) of VSV-G fusion protein in 293 cells with red and counterstained with ...