VSV-G (Vesicular stomatitis virus G protein)是一种用于分子生物学和生物医学研究中的实验工具,其主要用途为将外源基因导入目标细胞中。VSV-G膜融合机制是指VSV-G通过与细胞膜融合,将载有目标基因的质粒转运进入细胞的过程。 VSV-G是一种质膜蛋白,包含有融合活性的抗原ic及针对ic的抗体。当将载有目标基因的...
该回答已被折叠 VSV-G蛋白(Vesicular Stomatitis Virus G protein)是病毒颗粒的包膜蛋白,它可以形成病毒颗粒的包膜。VSV-G蛋白之所以具有这样的能力,是因为它在病毒复制中发挥了重要的作用。 病毒在感染细胞时,需要进入细胞内部并释放其基因组。为了完成这一过程,病毒需要一个途径,即通过与细胞表面的受体结合来进入细...
A chimeric protein that is a fusion construct of a series of functional domains is used to deliver a therapeutic agent to a human subject suffering from disease. In some embodiments, the chimeric protein includes a therapeutic region, a transportation region, and a cleavage region disposed between...
VSV-G标签通常融合于目的蛋白的N-或者C-端,以便使用免疫组化方法来进行观察和分析。 Fig.1.Immunofluorescence staining (1:1,000) of VSV-G fusion protein in 293 cells with red and counterstained with DAPI. Fig.2.IP (1:200) - WB (1:5,000) analysis of VSV-G Tag fusion protein expression...
The N inth Peopleπs Hospital, School ofM edicine, Shanghai Jiaotong University, Shanghai 200011, China)Abstract: O bjective To obtain high titer ofpseudotyped retroviruses containing GFP gene coated with vesicular stomatitis virus G gly2coprotein(VSV2G) , which could be effectively concentrated ...
VSV-G is a vesicular stomatitis virus G (VSV-G) protein fragment. VSV-G protein is commonly used in biomedical research to pseudotype retroviral and lentiviral vectors, conveying the ability to transduce a broad range of mammalian cell types with genes of interest. ...
摘要:目的通过与疱疹性口腔炎病毒糖蛋白G基因(VSV2G)共转染,使含绿色荧光蛋白(GFP)基因的逆转录病毒经超速离 心得以浓缩,进一步提高GFP逆转录病毒的细胞感染效率。方法应用GFP2RV质粒转染PT67细胞,并用G418筛选出GFP阳 性克隆,利用其产生的病毒上清液再转染GP22293细胞,在G418筛选的同时,瞬时转染VSV2G基因。收集该...
VSV-G is a vesicular stomatitis virus G (VSV-G) protein fragment. VSV-G protein is commonly used in biomedical research to pseudotype retroviral and lentiviral vectors, conveying the ability to transduce a broad range of mammalian cell types with genes of interest. ...
VSV-G tag sequence (YTDIEMNRLGK) on VSV-G tagged fusion proteins when expressed N- or C-terminal to the fusion protein. Recognizes native and denatured forms of VSV-G tagged proteins. 免疫原 KLH标记的含有水泡性口炎病毒糖蛋白(VSV-G)的15个羧基末端氨基酸(497-511)的合成肽。
VSV-G,来源于水泡性口炎病毒的融合性外壳G糖蛋白,常被用于逆转录病毒和慢病毒载体的生物医学研究。VSV-G标签通常融合于目的蛋白的N-或者C-端,以便使用免疫组化方法来进行观察和分析。 Fig.1.Immunofluorescence staining (1:1,000) of VSV-G fusion protein in 293 cells with red and counterstained with ...