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Analytes were quantified using TargetLynx V4.1 software (Waters) by comparing sample peaks to a 12-point standard curve of IS (0–1200 μM), pCS (0–300 μM), pCG (0–2000 μM), HA (0–8000 μM), betaine (0–700 μM), choline (0–700 μM), carnitine (0–300 μM), and ...
with the lowest detection limit of 6 pg mL−1. This microfluidic device was specific to IFN-γin the presence of overabundant serum proteins and allowed the continuous monitoring of IFN-γ without adding exogenous reagents. It provided a universal point-of-care biosensing platform for the ...
whereas still leaving a small opening at the contact point of the particle to the glass slide. After that, the Janus micromotors were coated with 120 µL of 1% (w/v) PLGA (Sigma-Aldrich, P2191) prepared in ethyl acetate (Sigma-Aldrich, 270989) and containing 40 mg mL−1CLR...
A 10-μL aliquot of the peptide solution was added to 930 μL CSF to which blood was added at dif- ferent final concentrations (0, 0.05, 0.5, 5% (v/v)) followed by incubation in room temperature overnight at room temperature. IP-MS was conducted as described above. In vitro test ...
To pinpoint the origin of the observed proteolytic activity, blood was fractionated into leukocytes, thrombocytes, thrombocyte-free plasma, and erythrocytes. The fractions were spiked with Aβ1-40, incubated as described above, and analyzed by IP-MALDI MS (Fig. 4). While minor degradation ...
We found that the interspecies conservation levels45 are strongly increased at the interaction sites and the peak of conservation precisely pinpointed the junction of the two RNA fragments (Fig. 4e). When interacting with lincRNAs, pseudogene RNAs, transposon RNAs or other mRNAs, the interaction ...
This test measures the electrode potential at some point during a current pulse and applies Ohm’s law to calculate a resistance value. An early publication using this technique measured the potential at the end of a current pulse and argued that in vivo changes in response were initially due ...
Finally, we selected the entire hippocampus from each of three non-consecutive brain sections/time point and counted the number of TUNEL-positive neurons. 4.4. Immunofluorescence Localization of LC3 and AIF The rats were sacrificed after anesthesia, and perfused transcardially with 4% PFA, at differ...
Thermal and mechanical damage was found around the closure point of all RFVS devices specimens. The tissue seemed compressed and significantly elongated, with altered architecture. The mucosal architecture was destroyed, resulting in a thermal coagulum with holes in the tissue and no discernible ...