The in vitro pull-down assay is a well-established method to confirm direct binding in protein-protein interactions that was inferred from other interaction assays, such as two-hybrid analysis (1). The assay is usually carried out using glutathione-S-transfer ase-tagged or His-tagged fusion pro...
Here we describe an RNA-protein pull-down assay, which enables the identification of proteins that interact with an RNA under study. As an example we describe pull-down of proteins interacting with ncRNA XIST, which assists in the recruitment of the polycomb-repressive complex-2 (PRC2) and ...
GTPases Rab4 and Rab5, we have applied protein interaction assays using the yeast two-hybrid system and a glutathione S-transferase pull-down assay. We... Elena,Korobko,Sergey,... - 《Febs Journal》 被引量: 16发表: 2005年 ENDOSOMAL RAB EFFECTOR WITH PX-DOMAIN, an interacting partner of...
We also demonstrate by in vitro pull-down assays that the 72-kDa IE1 protein can specifically interact with the DNA binding domain of E2F1 (amino ... MJ Margolis,S Pajovic,EL Wong,... - 《Journal of Virology》 被引量: 183发表: 1995年 Human Mob Proteins Regulate the NDR1 and NDR2 ...
Thus, we performed in vitro GST pull-down assay to examine the interaction between these proteins in the presence or absence of TRAF2 (Fig. 6A). Lysates of 293 cells expressing HA-RIP and Flag-TRAF2 were incubated with GST-c-IAP2 or GST-C2C together with glutathione–Sepharose beads at ...
To test biotinylation of the ssDNA with interest for further bioconjugation or pull-down assays, Biotin-16-dCTP were added to the dNTPs mix up to 100% replacement of the canonical dCTP. To test backbone-modified nucleic acid incorporation with specific application in polymer stability, Alpha Thiol...
Whole-tumor lysates were analyzed by western blot analysis or pull down assays. (f) A hypothetical model of AT-101-induced lethality in leukemia cells. In this model, AT-101 induces RhoA/ROCK1 activation, leading to PTEN activation and Akt inactivation, which in turn results in Mcl-1 ...
Co-immunoprecipitation (Co-IP) and/or pull-down assays associated with mass spectrometry (MS) are most commonly used for this purpose. Both these approaches are very efficient for the recovery of protein complexes when the protein partners exhibit strong and stable interactions. In Co-IP, the ...
This interaction was further confirmed by in vitro GST pull-down and in vivo immunoprecipitation assays. Both Daxx and p73/p53 co-localized in nuclear ... K Eun‐Joo,P Jong‐Sup,U Soo‐Jong - 《Nucleic Acids Research》 被引量: 140发表: 2003年 Methylation of RASSF1A gene promoter is reg...
coli was confirmed to be in a native functional state by pull-down assay with nuclear extract of HEK293 cells showing that Ku70-HisTag possessed the ability to interact with endogenous Ku80 (Supplementary Figure 1e). In this experiment, Ku80 was not pulled down with Tau-HisTag as a ...