sion of the suppressor protein in this assay is transient and localized, which is in contrast to the persistent and systemic expression achieved through the use of virus vectors or from stably integrated transgenes. A critical consideration in assaying for silencing suppres- ...
Using and characterizing viral suppressor of RNAi to interfere with small RNA pathways in DrosophilaBassam BERRYChristophe ANTONIEWSKI
In this chapter, we describe a detailed protocol for an RNAi reporter assay in Drosophila S2 cells for the identification of VSR activity.关键词: Insect virus Antiviral immunity RNAi Viral suppressor of RNAi (VSR Reporter assay DOI: 10.1007/978-1-61779-037-9_12 被引量: 68 ...
ArticlelncRNA Sensing of a Viral Suppressor of RNAi Activates Non-canonical Innate Immune Signaling in Drosophila Author links open overlay panelLiqin Zhang 1 3, Wen Xu 1 3, Xinlei Gao 1, Wenjie Li 1, Shuishui Qi 1, Dongyang Guo 1, Olugbenga Emmanuel Ajayi 1, Shou-Wei Ding 2, Qingfa ...
RNA-silencing suppressors of geminiviruses Suppressor - siRNA - Geminivirus - PTGS - miRNA - SilencingGeminiviruses are single-stranded (ss) DNA viruses that not only cause... SM Ikegami - 《Journal of General Plant Pathology》 被引量: 53发表: 2008年 Development of plants resistant to tomato ...
et al. A virus-targeted plant receptor-like kinase promotes cell-to-cell spread of RNAi. Proc. Natl Acad. Sci. USA 115, 1388–1393 (2018). Article CAS PubMed PubMed Central Google Scholar Garnelo Gόmez, B. et al. The viral silencing suppressor P19 interacts with the receptor-like...
Plant viral diseases seriously restrict the yield and quality of crops. About 80% of the reported plant viruses are transmitted by vector insects. Elucidating the molecular signals and mechanisms of the trans-kingdom interactions between viruses, plants, and vector insects in the disease cycle of ...
Viral RNAi Suppressor Reversibly Binds siRNA to Outcompete Dicer and RISC via Multiple Turnover [ J ]. Journal of Molecular Biology, 2011, 408 (2): 262-276.Renata A. Rawlings,Vishalakshi Krishnan,Nils G. Walter.Viral RNAi Suppressor Reversibly Binds siRNA to Outcompete Dicer and RISC via ...
Coxsackievirus B3 (CVB3) carrying mutations in the viral 3A protein, previously proposed to be a virus-encoded suppressor of RNAi in another picornavirus, human enterovirus 71, had a strong replication defect in wildtype cells. However, this defect was not rescued in Argonaute-2 deficient cells,...
Moreover, we provide evidence that the RNA species targeted by Dicer-2 can be modulated by the presence of a viral suppressor of RNAi. This study uncovered several novel, heavily targeted features within viral genomes, offering insight into viral replication, viral immune evasion strategies, and ...