The replication cycle of a virus consists of a number of sequential steps involving the adsorption, penetration, replication of the parent virus, and release of progeny virus. The one-step growth curve provides valuable information regarding the replication cycle of a given virus in a given cell ...
Knowledge of viral replication strategies has provided insights into critical steps in the viral life cycle that can serve as potential targets for antiviral therapy. For example, drugs can be designed to interfere with virus binding to target cells or prevent penetration and disassembly once receptor...
(Fig.3c). As shown recently9, NP amino acid substitutions can cause a genome packaging defect and the release of viral particles which cannot initiate a full replication cycle (semi-infectious particles). To rule out the possibility of a packaging defect, we determined the relative ratio of ...
After the nucleic acid genome and other essential proteins are packaged within the capsid, which is assembled from one or several translated viral proteins, the final steps of virus replication occur: maturation and release. Maturation refers to the final changes withi...
Conventional capping of viral RNA Even when the viral replication cycle includes a nuclear phase, any virus entering a host cell must express its genes in the cytoplasm using the host translation machinery. Viral genomes can be made of single- stranded or double-stranded nucleic acids, either ...
[12,13]. Hence developing a cure for HBV infection is a priority. Recently, gene-based and combinatorial strategies targeting multiple steps in the HBV replication cycle have shown promise. The potential of gene-based strategies for eliminating cccDNA and reducing antigenemia has been demonstrated ...
cycle. Whereas some cellular functions are usurped by individual viruses or a few related viruses, others are more broadly exploited by unrelated viral families. The identification and characterization of host factors that promote replication of multiple viruses can contribute to better understanding ...
(36) markedly inhibited the CVA16 associated cytopathic effect and was reported to reduce CVA16 production by 3.5 and 6.0 logs at 3 and 5 mM, respectively. Time-of-drug analysis conducted by the author suggested that GA interferes with an early event of the CVA16 replication cycle (Wang et...
All characterized (+)RNA viruses assemble viral replication complexes, containing both viral and host proteins, on intracellular membranes. The subverted host factors play crucial parts in all steps of (+)RNA virus replication. Therefore, host factors are key determinants of viral pathology as well ...
Viral replicons capable of recapitulating various steps of the replication cycle without using a live virus could increase accessibility. Transfection of DNA- and RNA-based alphavirus replicons is commonly used to investigate viral replication and assess the efficacy of vaccines and antivirals12,17,...