The replication cycle of a virus consists of a number of sequential steps involving the adsorption, penetration, replication of the parent virus, and release of progeny virus. The one-step growth curve provides valuable information regarding the replication cycle of a given virus in a given cell ...
Knowledge of viral replication strategies has provided insights into critical steps in the viral life cycle that can serve as potential targets for antiviral therapy. For example, drugs can be designed to interfere with virus binding to target cells or prevent penetration and disassembly once receptor...
During the past decade, a number of drug candidates targeting hRSV entry12–16 or replication steps17–19 have been advanced to pre-clinical or clinical development. The hRSV genomic RNA (vRNA) is packaged by the viral nucleoprotein (N) at all times, forming a N:RNA complex, called ...
Other viruses, such as DENV and WNV, are targeted by a number of ISGs at multiple stages of the viral replication cycle65. ISGs can exert virus-specific activities; for example, IFN-α-inducible protein 6 was found to selectively inhibit replication of certain flaviviridae family viruses by ...
After the nucleic acid genome and other essential proteins are packaged within the capsid, which is assembled from one or several translated viral proteins, the final steps of virus replication occur: maturation and release. Maturation refers to the final changes within...
[12,13]. Hence developing a cure for HBV infection is a priority. Recently, gene-based and combinatorial strategies targeting multiple steps in the HBV replication cycle have shown promise. The potential of gene-based strategies for eliminating cccDNA and reducing antigenemia has been demonstrated ...
Bracoviruses represent the most complex endogenous viral elements (EVEs) described to date. Nudiviral genes have been hosted within parasitoid wasp genomes since approximately 100 Ma. They play a crucial role in the wasp life cycle as they produce bracovirus particles, which are injected into parasi...
Virus-Host Cell Interactions during Hepatitis C Virus RNA Replication: Impact of Polyprotein Expression on the Cellular Transcriptome and Cell Cycle Associ... 2004. Virus-host cell interactions during hepatitis C virus RNA replication: impact of polyprotein expression on the cellular transcrip- tome...
(Fig.3c). As shown recently9, NP amino acid substitutions can cause a genome packaging defect and the release of viral particles which cannot initiate a full replication cycle (semi-infectious particles). To rule out the possibility of a packaging defect, we determined the relative ratio of ...
In contrast, the level of CIB1 and CIB2 expression did not influence cell viability, cell proliferation, receptor-independent viral binding to the cell surface, or later steps in the viral replication cycle. CIB1 and CIB2 knockdown was found to reduce the expression of surface molecules ...