Some tools, such as BLAST, are not appropriate for this purpose as they were not developed for this task, and the researcher would need to spend a considerable amount of time to manually check each alignment and some relevant ones could easily be missed (Senthil-Kumar and Mysore, 2011). ...
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VIGS沉默片段预测网站:https://vigs.solgenomics.net/ 定量PCR引物设计网站:https://www.ncbi.nlm.nih.gov/tools/primer-blast/index.cgi?LINK_LOC=BlastHome 在线密码子优化网站:https://www.novopro.cn/tools/codon-optimization.html 参考文献:郝梦媛,杭琦,师恭曜.VIGS基因沉默技术在作物基因功能研究中的应用与...
targetregionsiscurrentlyavailable.Sometools,suchasBLAST, arenotappropriateforthispurposeastheywerenotdeveloped forthistask,andtheresearcherwouldneedtospendaconsider- ableamountoftimetomanuallycheckeachalignmentand somerelevantonescouldeasilybemissed(Senthil-Kumarand Mysore,2011).OthertoolssuchaspssRNAit(http://pl...
Gene Silencing: Hush Genes to Make Them Talk. Trends Plant Sci 25 (7):714-715. doi:10.1016/j.tplants.2020.02.013Deshmukh, R. K., Sonah, H., & Bélanger, R. R. (2016). Plant Aquaporins: Genome-Wide Identification, Transcriptomics, Proteomics, and Advanced Analytical Tools. Frontiers...
VIGS vectors for gene silencing: many targets, many tools. The discovery that plants recognize and degrade invading viral RNA caused a paradigm shift in our understanding of viral/host interactions. Combined with t... Robertson,Dominique - 《Annual Review of Plant Biology》 被引量: 547发表: 200...
Plant viruses serve as a suitable candidate for understanding functional genomics by their modification as Virus Induced Gene Silencing vectors. Recent advancements in genetic engineering tools have made a significant contribution to their use as vectors. Here in this chapter, we have tried to discuss...
首先,经生物信息学软件分析(https://www.genscript.com/tools/sirna-target-finder),分析确定现有病毒沉默载体cpb-cc-pds中pds片段中可能产生的sirna序列(序列如seqidno.1所示)为:5’-aagatggtttatcagtcaaag-3’; 然后,人工合成包含该序列的44bp的pds基因片段pds1-f,另外合成在3’端添加碱基“tac”的反向互补...
Summary RNA-interference (RNAi) is a sequence-specific gene-silencing mechanism that is triggered by introduction of a double-stranded RNA (dsRNA) by employing genetic engineering tools and techniques, which ultimately results in formation of small interfering RNAs (siRNAs), the key molecules of ...
4、gene function analysis recently. VIGS has many advantages compared to commonly used biotechnological tools such as gene transformation,gene knockout and antisense inhibition. It is rapid with low cost,no need for stable plant transformation,and allows a large-scale screening of genes. Consequently...